Nurliyana M, Amal M N A, Zamri-Saad M, Ina-Salwany M Y
Department of Biology, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia.
Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia.
Lett Appl Microbiol. 2019 Jun;68(6):485-496. doi: 10.1111/lam.13146. Epub 2019 Apr 25.
This study investigates the possible transmission routes of Vibrio spp. in a tropical cage-cultured marine fishes. Samplings of cultured Asian seabass, red snapper, hybrid grouper, wild fish, trash fish, fish fry, water and sediment samples were conducted from December 2016 to August 2017. All fish were dissected in situ and swabs were taken aseptically from the skin, eye, liver and kidney for bacterial isolation and identification. Bacterial isolation and identification from water, sediment and trash fish were also made. A total of 261 Vibrio spp. isolates recovered from the cultured, wild and fry fish, as well as from the sediment and water of the farm environment were analysed. Sequences of the pyrH gene were used to investigate the degree of relatedness and possible transmission routes existing between the isolated Vibrio spp. The population tree revealed the existence of selected Vibrio spp. that possibly transmitted between the newly introduced fish fry and wild fish into the cultured fish, while water also might possibly serves as natural transmission medium of certain Vibrio spp. in this fish farm. SIGNIFICANCE AND IMPACT OF THE STUDY: The source of transmission of Vibrio spp. into farmed marine fish remains unclear. This study highlights the possible transmission routes of Vibrio into cage-cultured marine fishes via newly introduced fish fry and wild fish. Understanding the routes of transmission of Vibrio spp. might help in controlling the disease in the near future.
本研究调查了热带网箱养殖海鱼中弧菌属的可能传播途径。于2016年12月至2017年8月对养殖的尖吻鲈、红笛鲷、杂交石斑鱼、野生鱼、杂鱼、鱼苗、水和沉积物样本进行了采样。所有鱼类均在现场解剖，并无菌采集皮肤、眼睛、肝脏和肾脏的拭子用于细菌分离和鉴定。还对水、沉积物和杂鱼进行了细菌分离和鉴定。对从养殖鱼、野生鱼和鱼苗以及养殖场环境的沉积物和水中分离出的总共261株弧菌属菌株进行了分析。利用pyrH基因序列研究分离出的弧菌属之间的亲缘关系程度和可能的传播途径。种群树显示存在某些弧菌属，它们可能在新引入的鱼苗和野生鱼与养殖鱼之间传播，而水也可能是该养鱼场中某些弧菌属的自然传播媒介。研究的意义和影响：弧菌属传播到养殖海鱼中的来源尚不清楚。本研究强调了弧菌属通过新引入的鱼苗和野生鱼传播到网箱养殖海鱼中的可能途径。了解弧菌属的传播途径可能有助于在不久的将来控制疾病。
