The Joint Key Laboratory of Aquatic Products Processing of Zhejiang Province, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China.
Aquatic Products Quality Inspection Center of Zhejiang Province, Hangzhou 310012, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Apr 1;1112:11-15. doi: 10.1016/j.jchromb.2019.02.025. Epub 2019 Feb 25.
Hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS) was used as an accurate and sensitivity technique to separate and characterize the phospholipids in three strains of soft-shelled turtles. Under the optimized experimental parameters, the lipidomic profiles were successfully acquired, and a total of 55 phospholipid molecular species were identified. The principal component analysis (PCA) was applied to normalize the relative amounts of the phospholipid molecular species and revealed the differences among three strains of soft-shelled turtle, including Qingxi Huabie (QXH, GS-01-003-2008), Zhexin Wubie (ZXW, GS-02-005-2015), and Zhewu Erhao (ZWE, unregistered). Comparing with other methods, HILIC-MS is rapid, efficient and suitable in determining and visualizing phospholipids. This method also can be used for authenticating different strains of soft-shelled turtles.
亲水相互作用液相色谱-质谱联用（HILIC-MS）被用作一种准确和灵敏的技术，用于分离和鉴定三种鳖种的磷脂。在优化的实验参数下，成功获得了脂质组学图谱，共鉴定出 55 种磷脂分子种类。主成分分析（PCA）用于归一化磷脂分子种类的相对丰度，揭示了三种鳖种之间的差异，包括清溪花鳖（QXH，GS-01-003-2008）、浙新乌鳖（ZXW，GS-02-005-2015）和浙武二号（ZWE，未注册）。与其他方法相比，HILIC-MS 快速、高效，适合于确定和可视化磷脂。该方法也可用于鉴定不同鳖种。
