Yoshihara T, Kanda T, Yaku Y, Nagata H, Kaneko T, Tatsuoka H
Acta Otolaryngol. 1986 Jan-Feb;101(1-2):96-101.
We demonstrated the ultrastructures of the nasal mucosa of the rat by rapid freezing and freeze substitution with OsO4. Liquid helium was used for the rapid freezing. The depth of the good preservation was approximately 20 micron below the cut surface of the tissue. The characteristic features of the nasal mucosa obtained here are as follows: 1) The cross-section profiles of the cilium were round and smooth. 2) Mitochondrial matrix was electron dense and a few dense granules were observed in it. 3) Secretory granules of the goblet cells were enclosed by a limited membrane. 4) Extracellular space was of variable widths. 5) Cytoplasmic filaments were well preserved.
我们通过快速冷冻并用四氧化锇进行冷冻置换来展示大鼠鼻黏膜的超微结构。使用液氦进行快速冷冻。良好保存的深度在组织切面以下约20微米处。在此获得的鼻黏膜的特征如下：1）纤毛的横截面轮廓呈圆形且光滑。2）线粒体基质电子密度高，其中观察到一些致密颗粒。3）杯状细胞的分泌颗粒被有限的膜包围。4）细胞外间隙宽度可变。5）细胞质细丝保存良好。
