Some Characteristics of Acid Injury and Recovery of Salmonella bareilly in a Model System.

The injury and recovery characteristics of Salmonella bareilly after treatment with acetic acid in saline solution containing 0.5% sucrose were studied. Injury and death rates were dependent upon acid concentration and temperature. Several enzyme activities were substantially reduced in injured cells. Recovery rates were greater in peptones and casamino acids than in several commonly used culture media. The effects of a variety of inhibitors and antimetabolites on recovery of acid-injured cells indicated that protein and RNA synthesis, as well as electron transport, were necessary for recovery.