Yoon Gwangho, Cho Kyung A, Song Juhyun, Kim Young-Kook
Department of Anatomy, Chonnam National University Medical School, Jeollanam-do, South Korea.
Department of Biochemistry, Chonnam National University Medical School, Jeollanam-do, South Korea.
Front Genet. 2019 Feb 19;10:83. doi: 10.3389/fgene.2019.00083. eCollection 2019.
High fat diet can lead to metabolic diseases such as obesity and diabetes known to be chronic inflammatory diseases with high prevalence worldwide. Recent studies have reported cognitive dysfunction in obese patients is caused by a high fat diet. Accordingly, such dysfunction is called "type 3 diabetes" or "diabetic dementia." Although dysregulation of protein-coding genes has been extensively studied, profiling of non-coding RNAs including long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) has not been reported yet. Therefore, the objective of this study was to obtain profiles of diverse RNAs and determine their patterns of alteration in high fat fed brain cortex compared to normal brain cortex. To investigate regulatory roles of both coding and non-coding RNAs in high fat diet brain, we performed RNA sequencing of ribosomal RNA-depleted RNAs and identified genome-wide lncRNAs and circRNAs expression and co-expression patterns of mRNAs in high fat diet mouse brain cortex. Our results showed expression levels of mRNAs related to neurogenesis, synapse, and calcium signaling were highly changed in high fat diet fed cortex. In addition, numerous differentially expressed lncRNAs and circRNAs were identified. Our study provides valuable expression profiles and potential function of both coding and non-coding RNAs in high fat diet fed brain cortex.
高脂肪饮食会导致代谢性疾病，如肥胖症和糖尿病，这些疾病是全球范围内普遍存在的慢性炎症性疾病。最近的研究报告称，肥胖患者的认知功能障碍是由高脂肪饮食引起的。因此，这种功能障碍被称为“3型糖尿病”或“糖尿病性痴呆”。尽管对蛋白质编码基因的失调已经进行了广泛研究，但包括长链非编码RNA（lncRNA）和环状RNA（circRNA）在内的非编码RNA的分析尚未见报道。因此，本研究的目的是获得不同RNA的图谱，并确定与正常脑皮质相比，高脂喂养的脑皮质中它们的变化模式。为了研究编码和非编码RNA在高脂饮食脑中的调控作用，我们对去除核糖体RNA的RNA进行了RNA测序，并确定了高脂饮食小鼠脑皮质中全基因组lncRNA和circRNA的表达以及mRNA的共表达模式。我们的结果表明，在高脂饮食喂养的皮质中，与神经发生、突触和钙信号相关的mRNA表达水平发生了高度变化。此外，还鉴定出了许多差异表达的lncRNA和circRNA。我们的研究提供了高脂饮食喂养的脑皮质中编码和非编码RNA的有价值的表达图谱和潜在功能。
