Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China.
BMC Genomics. 2018 May 10;19(1):356. doi: 10.1186/s12864-018-4754-2.
Cryptosporidium baileyi is the most common Cryptosporidium species in birds. However, effective prevention measures and treatment for C. baileyi infection were still not available. Long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) play important roles in regulating occurrence and progression of many diseases and are identified as effective biomarkers for diagnosis and prognosis of several diseases. In the present study, the expression profiles of host mRNAs, lncRNAs and circRNAs associated with C. baileyi infection were investigated for the first time.
The tracheal tissues of experimental (C. baileyi infection) and control chickens were collected for deep RNA sequencing, and 545,479,934 clean reads were obtained. Of them, 1376 novel lncRNAs were identified, including 1161 long intergenic non-coding RNAs (lincRNAs) and 215 anti-sense lncRNAs. A total of 124 lncRNAs were found to be significantly differentially expressed between the experimental and control groups. Additionally, 14,698 mRNAs and 9085 circRNAs were identified, and significantly different expressions were observed for 1317 mRNAs and 104 circRNAs between two groups. Bioinformatic analyses of gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway for their targets and source genes suggested that these dysregulated genes may be involved in the interaction between the host and C. baileyi.
The present study revealed the expression profiles of mRNAs, lncRNAs and circRNAs during C. baileyi infection for the first time, and sheds lights on the roles of lncRNAs and circRNAs underlying the pathogenesis of Cryptosporidium infection.
Crypto sporidium baileyi 是鸟类中最常见的 Crypto sporidium 物种。然而，针对 C. baileyi 感染，仍然没有有效的预防措施和治疗方法。长链非编码 RNA（lncRNA）和环状 RNA（circRNA）在调节许多疾病的发生和发展中发挥着重要作用，并被鉴定为多种疾病诊断和预后的有效生物标志物。本研究首次研究了与 C. baileyi 感染相关的宿主 mRNA、lncRNA 和 circRNA 的表达谱。
收集实验组（C. baileyi 感染）和对照组鸡的气管组织进行深度 RNA 测序，共获得 545,479,934 条清洁读段。其中，鉴定出 1376 个新型 lncRNA，包括 1161 个长基因间非编码 RNA（lincRNA）和 215 个反义 lncRNA。实验组和对照组之间共发现 124 个 lncRNA 表达显著差异。此外，鉴定出 14,698 个 mRNAs 和 9085 个 circRNAs，两组间有 1317 个 mRNAs 和 104 个 circRNAs 表达显著差异。对其靶基因和来源基因的基因本体（GO）和京都基因与基因组百科全书（KEGG）通路的生物信息学分析表明，这些失调基因可能参与宿主与 C. baileyi 的相互作用。
本研究首次揭示了 C. baileyi 感染过程中 mRNA、lncRNA 和 circRNA 的表达谱，为了解 Crypto sporidium 感染的发病机制中 lncRNA 和 circRNA 的作用提供了线索。
