Jung Chan Woo, Lee Jae Sang, Jalani Ghulam, Hwang Eun Young, Lim Dong Woo
Department of Bionano Engineering and Bionanotechnology, College of Engineering Sciences, Hanyang University, Ansan, South Korea.
Front Chem. 2019 Feb 19;7:73. doi: 10.3389/fchem.2019.00073. eCollection 2019.
Stimuli-responsive anisotropic microstructures and nanostructures with different physicochemical properties in discrete compartments, have been developed as advanced materials for drug delivery systems, tissue engineering, regenerative medicine, and biosensing applications. Moreover, their stimuli-triggered actuations would be of great interest for the introduction of the functionality of drug delivery reservoirs and tissue engineering scaffolds. In this study, stimuli-responsive bicompartmental nanofibers (BCNFs), with completely different polymer compositions, were prepared through electrohydrodynamic co-jetting with side-by-side needle geometry. One compartment with thermo-responsiveness was composed of methacrylated poly(N-isopropylacrylamide-co-allylamine hydrochloride) (poly(NIPAM-co-AAh)), while the counter compartment was made of poly(ethylene glycol) dimethacrylates (PEGDMA). Both methacrylated poly(NIPAM-co-AAh) and PEGDMA in distinct compartments were chemically crosslinked in a solid phase by UV irradiation and swelled under aqueous conditions, because of the hydrophilicity of both poly(NIPAM-co-AAh) and PEGDMA. As the temperature increased, BCNFs maintained a clear interface between compartments and showed thermally-induced actuation at the nanoscale due to the collapsed poly(NIPAM-co-AAh) compartment under the PEGDMA compartment of identical dimensions. Different model drugs, bovine serum albumin, and dexamethasone phosphate were alternately loaded into each compartment and released at different rates depending on the temperature and molecular weight of the drugs. These BCNFs, as intelligent nanomaterials, have great potential as tissue engineering scaffolds and multi-modal drug delivery reservoirs with stimuli-triggered actuation and decoupled drug release.
在离散隔室中具有不同物理化学性质的刺激响应性各向异性微结构和纳米结构，已被开发为用于药物递送系统、组织工程、再生医学和生物传感应用的先进材料。此外，它们的刺激触发驱动对于引入药物递送储库和组织工程支架的功能将具有极大的吸引力。在本研究中，通过采用并排针状几何结构的电流体动力学共喷射制备了具有完全不同聚合物组成的刺激响应性双隔室纳米纤维（BCNFs）。一个具有热响应性的隔室由甲基丙烯酸化的聚（N-异丙基丙烯酰胺-共-烯丙胺盐酸盐）（聚（NIPAM-共-AAh））组成，而相对的隔室则由聚（乙二醇）二甲基丙烯酸酯（PEGDMA）制成。不同隔室中的甲基丙烯酸化聚（NIPAM-共-AAh）和PEGDMA在固相通过紫外线照射进行化学交联，并在水性条件下膨胀，这是由于聚（NIPAM-共-AAh）和PEGDMA都具有亲水性。随着温度升高，BCNFs在隔室之间保持清晰的界面，并由于相同尺寸的PEGDMA隔室下方聚（NIPAM-共-AAh）隔室的塌陷而在纳米尺度上表现出热诱导驱动。不同的模型药物、牛血清白蛋白和磷酸地塞米松被交替加载到每个隔室中，并根据药物的温度和分子量以不同速率释放。这些BCNFs作为智能纳米材料，具有作为具有刺激触发驱动和解耦药物释放的组织工程支架和多模式药物递送储库的巨大潜力。
