Safari Sogand, Ehsani Morteza, Zandi Mojgan
Department of Polymer Processing, Iran Polymer and Petrochemical Institute, Tehran, Iran.
Department of Biomaterials, Iran Polymer and Petrochemical Institute, Tehran, Iran.
Prog Biomater. 2021 Dec;10(4):245-258. doi: 10.1007/s40204-021-00168-1. Epub 2021 Nov 3.
Poly(N-vinylcaprolactam) (PNVCL) is a suitable alternative for biomedical applications due to its biocompatibility, biodegradability, non-toxicity, and showing phase transition at the human body temperature range. The purpose of this study was to synthesize a high molecular weight PNVCL-PVAc thermo-responsive copolymer with broad mass distribution suitable for electrospun nanofiber fabrication. The chemical structure of the synthesized materials was detected by FTIR and HNMR spectroscopies. N-Vinyl caprolactam/vinyl acetate copolymers (159,680 molecular weight (g/mol) and 2.51 PDI) were synthesized by radical polymerization. The phase transition temperature of N-vinyl caprolactam/vinyl acetate copolymer was determined by conducting a contact angle test at various temperatures (25, 26, 28, and 30 [Formula: see text]). The biocompatibility of the nanofibers was also evaluated, and both qualitative and quantitative results showed that the growth and proliferation of 929L mouse fibroblast cells increased to 80% within 48 h. These results revealed that the synthesized nanofibers were biocompatible and not cytotoxic. The results confirmed that the synthesized copolymers have good characteristics for biomedical applications.
聚（N-乙烯基己内酰胺）（PNVCL）因其生物相容性、生物可降解性、无毒性以及在人体体温范围内呈现相变特性，是生物医学应用的合适替代品。本研究的目的是合成一种具有宽质量分布的高分子量PNVCL-PVAc热响应共聚物，以适用于电纺纳米纤维的制备。通过傅里叶变换红外光谱（FTIR）和核磁共振氢谱（HNMR）对合成材料的化学结构进行了检测。通过自由基聚合合成了N-乙烯基己内酰胺/醋酸乙烯酯共聚物（分子量为159,680 g/mol，多分散指数为2.51）。通过在不同温度（25、26、28和30 [公式：见原文]）下进行接触角测试，测定了N-乙烯基己内酰胺/醋酸乙烯酯共聚物的相变温度。还评估了纳米纤维的生物相容性，定性和定量结果均表明，929L小鼠成纤维细胞在48小时内的生长和增殖增加到了80%。这些结果表明合成的纳米纤维具有生物相容性且无细胞毒性。结果证实，合成的共聚物具有良好的生物医学应用特性。
