用于上皮-间质相互作用研究的组织重组和肾包膜移植试验

Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-Mesenchymal Interactions.

作者信息

Jimenez-Rojo Lucia, Mitsiadis Thimios A

机构信息

Faculty of Medicine, Orofacial Development and Regeneration, Institute of Oral Biology, Center of Dental Medicine, ZZM, University of Zurich, Zurich, Switzerland.

出版信息

Methods Mol Biol. 2019;1922:49-55. doi: 10.1007/978-1-4939-9012-2_6.

DOI:10.1007/978-1-4939-9012-2_6

PMID:30838564

Abstract

Tissue interactions are crucial during the development of organs. Among the most studied tissue interactions are those that take place between the epithelial cells and the underlying mesenchymal cells, known as epithelial-mesenchymal interactions. Tissue recombination assay is a valuable model to study the mechanisms involved in the regulation of such interactions. Here, we describe how to dissociate and recombine the epithelial and mesenchymal components of the embryonic tooth. In addition, we explain how to transplant the recombined tissues under the kidney capsule of immunocompromised mice in order to allow their further development into a mature tooth.

摘要

组织间相互作用在器官发育过程中至关重要。上皮细胞与下方间充质细胞之间发生的相互作用是研究最多的组织间相互作用之一，称为上皮-间充质相互作用。组织重组实验是研究此类相互作用调控机制的一种有价值的模型。在此，我们描述如何分离和重组胚胎牙齿的上皮和间充质成分。此外，我们解释如何将重组组织移植到免疫缺陷小鼠的肾被膜下，以便使其进一步发育成一颗成熟的牙齿。