Kechagioglou Petros, Andriotis Eleftherios, Papagerakis Petros, Papagerakis Silvana
Department of Surgery, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
College of Dentistry, University of Saskatchewan, Saskatoon, SK, Canada.
Methods Mol Biol. 2019;1922:121-128. doi: 10.1007/978-1-4939-9012-2_12.
Multiwalled carbon nanotubes (MWCNTs) are a particularly promising drug delivery system due to their high surface area allowing high-protein loading, their stability under biological conditions, and their unique interaction with cellular membranes. Studies have shown that covalent attachment of polyethylene glycol (PEG) improves biocompatibility and enhances surface hydrophilicity properties, suggesting that PEGylated MWCNTs are efficient and toxic-safe drug delivery systems. So far, CNTs are used for a broad range of applications in dentistry, especially for dental tissue repair and restorative. Here we present a protocol of protein immobilization onto MWCNTs and describe the procedure for delivering them into the cells after characterization of the nanotubes.
多壁碳纳米管（MWCNTs）是一种特别有前景的药物递送系统，因为它们具有高表面积，能够实现高蛋白负载，在生物条件下具有稳定性，并且与细胞膜有独特的相互作用。研究表明，聚乙二醇（PEG）的共价连接可改善生物相容性并增强表面亲水性，这表明聚乙二醇化的多壁碳纳米管是高效且毒性安全的药物递送系统。到目前为止，碳纳米管在牙科领域有广泛的应用，特别是用于牙科组织修复和修复。在此，我们展示了一种将蛋白质固定到多壁碳纳米管上的方案，并描述了在对纳米管进行表征后将它们递送至细胞中的程序。
