Joshi Amar, Zahoor Amna, Buson Alberto
Pharmaxis Drug Discovery, Frenchs Forest, NSW, Australia.
Methods Mol Biol. 2019;1944:79-93. doi: 10.1007/978-1-4939-9095-5_6.
All tissues contain an extracellular matrix (ECM) which is constantly and dynamically remodeled, either in physiological or pathological processes, such as fibrosis or cancer. One of the key contributors in the establishment of a fibrotic state is the abnormal deposition of extracellular matrix and cross-linked proteins, in particular collagen, leading to tissue stiffening and disruption of organ function. The precise and sensitive measurement of these cross-links by LC-MS/MS is a very powerful tool for providing a quantitative and qualitative analysis of fibrosis and is a key requirement in the study of this state, as well as in the development of drugs for this unmet clinical need.
所有组织都含有细胞外基质（ECM），在生理或病理过程中，如纤维化或癌症，该基质会不断且动态地重塑。纤维化状态形成的关键因素之一是细胞外基质和交联蛋白（尤其是胶原蛋白）的异常沉积，这会导致组织硬化和器官功能紊乱。通过液相色谱-串联质谱法（LC-MS/MS）精确且灵敏地测量这些交联是对纤维化进行定量和定性分析的强大工具，也是研究这种状态以及开发针对这一未满足临床需求的药物的关键要求。
