Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Eur Rev Med Pharmacol Sci. 2019 Feb;23(4):1388-1396. doi: 10.26355/eurrev_201902_17095.
The aim of this study was to investigate whether lnc00908 could affect the proliferative and migratory behaviors of ovarian cancer (OC) cells by regulating microRNA-495-5p, thus participating in the development of OC.
Quantitative Real Time-Polymerase Chain Reaction (QRT-PCR) was used to detect the expression levels of lnc00908 and microRNA-495-5p in OC tissues and normal ovarian tissues, as well as OC cell lines. The regulatory effects of lnc00908 and microRNA-495-5p on the proliferative and migration abilities of OC cells were detected by cell counting kit-8 (CCK-8) and transwell assay, respectively. The binding relationship between microRNA-495-5p and ANXA3, as well as miR-495-5p and lnc00908, was examined by luciferase reporter gene assay. Gain-of-function experiments were conducted to verify whether lnc00908 could affect the proliferative and migratory behaviors of OC cells by regulating microRNA-495-5p.
Lnc00908 was highly expressed in OC tissues, and its expression was positively correlated with tumor stage. Overexpression of lnc00908 markedly promoted the proliferative and migratory abilities of SKOV3 and OVCAR cells. Luciferase reporter gene assay showed that lnc00908 could bind to microRNA-495-5p. However, microRNA-495-5p was significantly downregulated in OC tissues. Overexpression of microRNA-495-5p reversed the enhanced abilities of proliferation and migration in SKOV3 and OVCAR3 cells by lnc00908 overexpression. ANXA3 was a target gene of microRNA-495-5p. Moreover, overexpression of ANXA3 attenuated the inhibitory effect of miR-495-5p on the proliferative and migratory behaviors of SKOV3 and OVCAR3 cells.
We found that the high expression of lnc00908 can promote the proliferation and migration abilities of OC cells through sponging microRNA-495-5p to regulate ANXA3 expression.
本研究旨在探讨 lnc00908 是否通过调控 microRNA-495-5p 影响卵巢癌（OC）细胞的增殖和迁移行为，从而参与 OC 的发生发展。
采用实时定量聚合酶链反应（QRT-PCR）检测 OC 组织及正常卵巢组织、OC 细胞系中 lnc00908 和 microRNA-495-5p 的表达水平，通过细胞计数试剂盒-8（CCK-8）和 Transwell 实验分别检测 lnc00908 和 microRNA-495-5p 对 OC 细胞增殖和迁移能力的调控作用，采用荧光素酶报告基因实验检测 microRNA-495-5p 与 ANXA3 以及 miR-495-5p 与 lnc00908 的结合关系，通过 gain-of-function 实验验证 lnc00908 是否通过调控 microRNA-495-5p 影响 OC 细胞的增殖和迁移行为。
lnc00908 在 OC 组织中高表达，且其表达与肿瘤分期呈正相关。过表达 lnc00908 显著促进 SKOV3 和 OVCAR 细胞的增殖和迁移能力。荧光素酶报告基因实验显示，lnc00908 可与 microRNA-495-5p 结合。然而，OC 组织中 microRNA-495-5p 表达显著下调。过表达 microRNA-495-5p 逆转了 lnc00908 过表达对 SKOV3 和 OVCAR3 细胞增殖和迁移能力的增强作用。ANXA3 是 microRNA-495-5p 的靶基因。此外，过表达 ANXA3 减弱了 miR-495-5p 对 SKOV3 和 OVCAR3 细胞增殖和迁移行为的抑制作用。
我们发现 lnc00908 的高表达通过海绵吸附 microRNA-495-5p 来调节 ANXA3 的表达，从而促进 OC 细胞的增殖和迁移能力。
