Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Wuhan, China.
Eur Rev Med Pharmacol Sci. 2019 Feb;23(4):1494-1501. doi: 10.26355/eurrev_201902_17107.
To investigate the role and molecular mechanism of miR-182-5p in the development of colorectal cancer (CRC), thereby providing a theoretical basis for new CRC therapeutic targets.
The expression of miR-182-5p in CRC clinical cases and biological cell lines was detected. On-line target gene prediction and Luciferase reporter gene assay were performed to screen and verify the target of miR-182-5p, respectively. The role of miR-182-5p in CRC cell function was further analyzed.
MiR-182-5p expression was significantly decreased in both CRC tissues and cell lines. Metadherin (MTDH) was screened and verified as a functional target of miR-182-5p. The cell proliferation, invasion and migration ability of CRC cells were significantly inhibited after the up-regulation of miR-182-5p. However, MTDH limited the anti-cancer effects of miR-182-5p in CRC cells.
Our research demonstrated the inhibitory function of miR-182-5p in CRC. Therefore, the miR-182-5p/MTDH axis was expected to be one of the targets of CRC targeted therapy.
研究 miR-182-5p 在结直肠癌（CRC）发展中的作用和分子机制，为新的 CRC 治疗靶点提供理论依据。
检测 CRC 临床病例和生物细胞系中 miR-182-5p 的表达。通过在线靶基因预测和荧光素酶报告基因实验，分别筛选和验证 miR-182-5p 的靶基因。进一步分析 miR-182-5p 对 CRC 细胞功能的作用。
miR-182-5p 在 CRC 组织和细胞系中的表达均显著降低。通过筛选和验证，发现调节素（MTDH）是 miR-182-5p 的功能靶基因。上调 miR-182-5p 可显著抑制 CRC 细胞的增殖、侵袭和迁移能力。然而，MTDH 限制了 miR-182-5p 在 CRC 细胞中的抗癌作用。
本研究证实了 miR-182-5p 在 CRC 中的抑制作用。因此，miR-182-5p/MTDH 轴有望成为 CRC 靶向治疗的靶点之一。
