a Department of Thoracic Surgery , the Fourth Hospital of Hebei Medical University , Shijiazhuang , China.
b Department of Clinical Laboratory , Hebei Medical University , Shijiazhuang , China.
Bioengineered. 2019 Dec;10(1):1-12. doi: 10.1080/21655979.2019.1586056.
This study is aimed to elucidate the mechanisms underlying the role of miR-485-5p in small cell lung cancer (SCLC). The expression of miR-485-5p were quantified with real time quantitative PCR and it was found that the level of miR-485-5p was lower in SCLC tissues than normal tissues. In cultured SCLC cell lines, overexpression of miR-485-5p reduced cell proliferation, migration, and invasion in vitro, whereas knockdown of miR-485-5p performed contrary. FLOT2 expression was obviously upregulated and negatively correlated with miR-485-5p expression level in SCLC tissues. Overexpression of miR-485-5p significantly inhibited the protein expression of flotillin-2 (FLOT2) in cultured SCLC cells. Luciferase reporter assay confirmed that FLOT2 was a direct target of miR-485-5p in SCLC cells. It is concluded that miR-485-5p, as a tumor suppressor, inhibits the growth and metastasis in SCLC by targeting FLOT2. Upregulation of miR-485-5p expression may be an attractive strategy for SCLC therapy.
本研究旨在阐明 miR-485-5p 在小细胞肺癌（SCLC）中作用的机制。通过实时定量 PCR 定量检测 miR-485-5p 的表达，发现 SCLC 组织中 miR-485-5p 的水平低于正常组织。在培养的 SCLC 细胞系中，miR-485-5p 的过表达减少了体外细胞的增殖、迁移和侵袭，而 miR-485-5p 的敲低则表现相反。FLOT2 在 SCLC 组织中的表达明显上调，并与 miR-485-5p 的表达水平呈负相关。在培养的 SCLC 细胞中过表达 miR-485-5p 可显著抑制 flotillin-2（FLOT2）的蛋白表达。荧光素酶报告基因检测证实 FLOT2 是 SCLC 细胞中 miR-485-5p 的直接靶标。结论：miR-485-5p 作为一种肿瘤抑制因子，通过靶向 FLOT2 抑制 SCLC 的生长和转移。上调 miR-485-5p 的表达可能是 SCLC 治疗的一种有吸引力的策略。
