Department of Chemistry & Nanoscience and Technology Research and Application Center, Canakkale Onsekiz Mart University Terzioglu Campus, 17100 Canakkale, Turkey.
Department of Chemistry, Istanbul Technical University, 34469 Maslak, Istanbul, Turkey.
Int J Biol Macromol. 2019 Jun 1;130:627-635. doi: 10.1016/j.ijbiomac.2019.03.025. Epub 2019 Mar 3.
We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media. Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a ~2.5-fold increase in their Young moduli, e.g., from 38 ± 1 to 99 ± 4 kPa at a HA:HNTs weight ratio of 1:2. Although HA:HNTs based cryogels were found to be blood compatible with 1.37 ± 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 ± 4.8. Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116). The cell studies revealed that an increased amount of HNT embedding into HA cryogels leads to an increase of MSC proliferation.
我们在此介绍了基于透明质酸（HA）和海泡石纳米管（HNTs）的各种组成的机械强度高且生物相容性好的冷冻凝胶复合材料的制备及其作为不同细胞生长介质支架的应用。单轴压缩试验表明，将 HNTs 掺入 HA 冷冻凝胶中可将其杨氏模量提高约 2.5 倍，例如，在 HA:HNTs 重量比为 1:2 时，杨氏模量从 38±1kPa 增加到 99±4kPa。尽管在 HA:HNTs 重量比为 1:2 时，HA:HNTs 基冷冻凝胶的溶血率为 1.37±0.11%，被认为是血液相容的，但它们的血栓形成指数为 17.3±4.8，具有血栓形成活性。值得注意的是，HA:HNTs 冷冻凝胶复合材料是大鼠间充质干细胞（MSC）、人宫颈癌细胞（HeLa）和人结肠癌细胞（HCT116）增殖的优异支架材料。细胞研究表明，HA 冷冻凝胶中嵌入 HNTs 的量增加会导致 MSC 增殖增加。
