Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, Tehran, Iran.
J Biomed Mater Res A. 2018 May;106(5):1276-1287. doi: 10.1002/jbm.a.36327. Epub 2018 Jan 23.
Modified halloysite nanotubes (HNT-NH and HNT-COOH) were synthesized by a coupling reaction with 3-aminopropyltriethoxysilane and maleic anhydride from hydroxyl groups of neat halloysite nanotubes (HNTs). Successful attachment of functional groups onto HNTs was evaluated by Fourier transform infrared and thermogravimetric analysis. X ray diffraction was used to study the crystalline structure of scaffolds and the formation of intercalated structure as a result of improved dispersion and decreased agglomeration of modified nanoparticles. Neat HNT, HNT-NH , and HNT-COOH were subsequently introduced into polycaprolactone/Pluronic P123 (PCL/P123) electrospun substrate. Morphology, thermodynamics, mechanics, and biocompatibility of resulted electrospun nanocomposites were evaluated. Nanofibers containing modified HNTs showed excellent mechanical performance and thermal stability in comparison with those containing neat HNTs. homogeneous dispersion of the modified HNTs and strong interfacial adhesion between nanotubes and polymer matrix can be considered for mentioned improvements. Ultraviolet-visible spectrophotometer was used to study diphenhydramine hydrochloride and diclofenac sodium release from nanocomposites containing drug loaded modified and neat HNTs. Nanocomposites containing drug loaded HNT-COOH exhibited prolonged release of drug molecules in comparison with that of neat HNTs. MTT assay reveled that PCL/P123/modified HNTs nanocomposites provide a suitable platform for cell growth where PCL/P123/HNT-NH can facilitate cell attachment through electrostatic interactions between negatively charged phospholipids bilayer membrane of cells and positively charged HNT-NH embedded in PCL/P123 substrate. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1276-1287, 2018.
经 3-氨丙基三乙氧基硅烷和马来酸酐与纯埃洛石纳米管（HNTs）上的羟基进行偶联反应合成了修饰的埃洛石纳米管（HNT-NH 和 HNT-COOH）。通过傅里叶变换红外和热重分析评估了功能基团成功接枝到 HNTs 上。X 射线衍射用于研究支架的结晶结构以及插层结构的形成，这是由于改性纳米粒子的分散性提高和团聚减少。随后将纯 HNT、HNT-NH 和 HNT-COOH 引入到聚己内酯/Pluronic P123（PCL/P123）静电纺丝基质中。评估了所得静电纺纳米复合材料的形态、热力学、力学和生物相容性。与含有纯 HNTs 的纳米纤维相比，含有修饰的 HNTs 的纳米纤维表现出优异的机械性能和热稳定性。可以认为修饰的 HNTs 的均匀分散和纳米管与聚合物基质之间的强界面粘附是产生这些改进的原因。使用紫外可见分光光度计研究了含有载药修饰和纯 HNTs 的纳米复合材料中盐酸苯海拉明和双氯芬酸钠的释放。与纯 HNTs 相比，含有载药 HNT-COOH 的纳米复合材料表现出药物分子的延长释放。MTT 测定表明，PCL/P123/修饰的 HNTs 纳米复合材料为细胞生长提供了合适的平台，其中 PCL/P123/HNT-NH 通过细胞带负电荷的磷脂双层膜与嵌入 PCL/P123 基质中的带正电荷的 HNT-NH 之间的静电相互作用促进细胞附着。 © 2018 Wiley Periodicals, Inc. J 生物材料研究杂志 A 部分：106A：1276-1287，2018。
