Blodgett J T, Stanosz G R
Former Postdoctoral Research Associate.
Associate Professor, Departments of Plant Pathology, and Forest Ecology and Management, University of Wisconsin-Madison, 1630 Linden Drive, Madison 53706-1598.
Plant Dis. 1999 Sep;83(9):853-856. doi: 10.1094/PDIS.1999.83.9.853.
Seedlings of Scot's pine varieties East Anglia and Austrian Hills, red pine, mugho pine variety Pumileo, Colorado blue spruce, Douglas-fir, and balsam fir were wounded and inoculated with water agar plugs colonized by isolates of the two random amplified polymorphic DNA (RAPD) marker groups (A and B) of Sphaeropsis sapinea. Isolates were obtained from hosts in Michi-gan, Minnesota, and Wisconsin. Symptom severity (distance from the inoculation site at which necrotic needles were present) resulting from inoculations with each group A isolate exceeded that from inoculations with each group B isolate on all hosts except Colorado blue spruce. Hosts varied considerably in their responses to group A isolates. Based on symptom severity, East Anglia Scot's pine was most susceptible and balsam fir was least susceptible when inoculated with group A isolates. The pathogen was recovered from both symptomatic and asymptomatic seedlings inoculated with isolates of either group. Results emphasize the importance of characterizing a RAPD marker group(s) of S. sapinea encountered in the field or used in research; the need for comparative evaluations of resistance among coniferous genera, species, and varieties to S. sapinea of both groups; and the potential for asymptomatic persistence of S. sapinea from both groups in or on several coniferous hosts.
对欧洲赤松品种东安格利亚和奥地利丘陵、美国红松、矮丛松品种普米利奥、科罗拉多蓝云杉、花旗松和香脂冷杉的幼苗进行创伤处理，并接种由松球壳孢菌的两个随机扩增多态性DNA（RAPD）标记组（A和B）的分离株定殖的水琼脂块。分离株取自密歇根州、明尼苏达州和威斯康星州的寄主。除科罗拉多蓝云杉外，在所有寄主上，用A组每个分离株接种导致的症状严重程度（出现坏死针叶的接种部位距离）超过用B组每个分离株接种导致的症状严重程度。寄主对A组分离株的反应差异很大。根据症状严重程度，接种A组分离株时，东安格利亚欧洲赤松最易感，香脂冷杉最不易感。从接种了两组分离株的有症状和无症状幼苗中均分离到了病原菌。结果强调了对田间遇到的或研究中使用的松球壳孢菌的RAPD标记组进行特征描述的重要性；对针叶树属、种和品种对两组松球壳孢菌的抗性进行比较评估的必要性；以及两组松球壳孢菌在几种针叶树寄主内或其上无症状持续存在的可能性。
