Richarme G, Heine H G
Eur J Biochem. 1986 Apr 15;156(2):399-405. doi: 10.1111/j.1432-1033.1986.tb09596.x.
The binding protein-dependent transport of galactose and maltose occurs at a reduced but significant rate in Escherichia coli cells which have undergone a mild toluenization. Dihydrolipoate and 3-acetyl-NAD produce a severalfold stimulation of these transports in the toluenized cells. In parallel to the stimulation of galactose and maltose transport by dihydrolipoate and 3-acetyl-NAD, there is a stimulation by galactose and maltose of lipoamide dehydrogenase activities which seem to be related to the binding-protein-dependent transport of these sugars. The lipoamide dehydrogenase component of the pyruvate and 2-oxoglutarate dehydrogenase complexes (the lpd gene product) is not involved in this stimulation. These results are discussed in relation to our recent studies showing a possible involvement of lipoic acid and of the 2-oxoacid dehydrogenases in the binding-protein-dependent transports.
在经过轻度甲苯处理的大肠杆菌细胞中，依赖结合蛋白的半乳糖和麦芽糖转运以降低但显著的速率发生。二氢硫辛酸和3-乙酰-NAD在经甲苯处理的细胞中对这些转运有几倍的刺激作用。与二氢硫辛酸和3-乙酰-NAD对半乳糖和麦芽糖转运的刺激作用平行的是，半乳糖和麦芽糖对硫辛酰胺脱氢酶活性有刺激作用，这似乎与这些糖的依赖结合蛋白的转运有关。丙酮酸和2-氧代戊二酸脱氢酶复合物的硫辛酰胺脱氢酶组分（lpd基因产物）不参与这种刺激作用。结合我们最近的研究讨论了这些结果，这些研究表明硫辛酸和2-氧代酸脱氢酶可能参与了依赖结合蛋白的转运。
