嗜盐古菌沃氏嗜盐碱杆菌的二氢硫辛酰胺脱氢酶：克隆基因的同源过表达
Dihydrolipoamide dehydrogenase from the halophilic archaeon Haloferax volcanii: homologous overexpression of the cloned gene.
作者信息
Jolley K A, Rapaport E, Hough D W, Danson M J, Woods W G, Dyall-Smith M L
机构信息
School of Biology and Biochemistry, University of Bath, United Kingdom.
出版信息
J Bacteriol. 1996 Jun;178(11):3044-8. doi: 10.1128/jb.178.11.3044-3048.1996.
Abstract
The gene encoding dihydrolipoamide dehydrogenase from the halophilic archaeon, Haloferax volcanii, has been subcloned and overexpressed in the parent organism by using the halophilic archaeal rRNA promoter. The recombinant protein has been purified to homogeneity and characterized with respect to its kinetic, molecular, and salt-dependent properties. A dihydrolipoamide dehydrogenase-minus mutant of H. volcanii has been created by homologous recombination with the subcloned gene after insertion of the mevinolin resistance determinant into the protein-coding region. To explore the physiological function of the dihydrolipoamide dehydrogenase, the growth properties of the mutant halophile have been examined.
摘要
来自嗜盐古菌沃氏嗜盐栖热菌（Haloferax volcanii）的二氢硫辛酰胺脱氢酶编码基因已被亚克隆，并通过使用嗜盐古菌rRNA启动子在亲本生物体中过表达。重组蛋白已被纯化至同质，并对其动力学、分子和盐依赖性特性进行了表征。通过将美伐他汀抗性决定簇插入蛋白质编码区后，利用亚克隆基因进行同源重组，构建了沃氏嗜盐栖热菌的二氢硫辛酰胺脱氢酶缺失突变体。为了探索二氢硫辛酰胺脱氢酶的生理功能，已对突变嗜盐菌的生长特性进行了研究。
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本文引用的文献
1
Studies on the nature and reactions of protein-bound lipoic acid.蛋白质结合硫辛酸的性质及反应研究。
J Biol Chem. 1958 May;232(1):143-58.
2
Cloning, sequencing, and expression in Escherichia coli of the gene coding for malate dehydrogenase of the extremely halophilic archaebacterium Haloarcula marismortui.嗜盐古细菌死海嗜盐菌苹果酸脱氢酶编码基因的克隆、测序及在大肠杆菌中的表达
Biochemistry. 1993 Apr 27;32(16):4308-13. doi: 10.1021/bi00067a020.
3
High expression in Escherichia coli of the gene coding for dihydrofolate reductase of the extremely halophilic archaebacterium Haloferax volcanii. Reconstitution of the active enzyme and mutation studies.
Eur J Biochem. 1993 Aug 15;216(1):199-203. doi: 10.1111/j.1432-1033.1993.tb18133.x.
4
Improved shuttle vectors for Haloferax volcanii including a dual-resistance plasmid.用于嗜盐栖热菌的改良穿梭载体，包括一种双抗性质粒。
Gene. 1994 Aug 19;146(1):117-21. doi: 10.1016/0378-1119(94)90844-3.
5
Determination of structure-antioxidant activity relationships of dihydrolipoic acid.
Methods Enzymol. 1994;234:454-61. doi: 10.1016/0076-6879(94)34116-8.
6
The mitochondrial glycine cleavage system. Unique features of the glycine decarboxylation.线粒体甘氨酸裂解系统。甘氨酸脱羧作用的独特特征。
Mol Cell Biochem. 1982 Jun 25;45(3):137-49. doi: 10.1007/BF00230082.
7
Dihydrolipoamide dehydrogenase from halophilic archaebacteria.嗜盐古细菌的二氢硫辛酰胺脱氢酶。
Biochem J. 1984 Mar 15;218(3):811-8. doi: 10.1042/bj2180811.
8
A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast.一种用于聚乙二醇诱导细菌和酵母遗传转化的通用方法。
Gene. 1983 Nov;25(2-3):333-41. doi: 10.1016/0378-1119(83)90238-x.
9
Dihydrolipoamide dehydrogenase from Trypanosoma brucei. Characterization and cellular location.布氏锥虫二氢硫辛酰胺脱氢酶。特性及细胞定位
Biochem J. 1987 May 1;243(3):661-5. doi: 10.1042/bj2430661.
10
Dihydrolipoamide dehydrogenase: a 'new' function for an old enzyme?二氢硫辛酰胺脱氢酶：一种古老酶的“新”功能？
Biochem Soc Trans. 1988 Apr;16(2):87-9. doi: 10.1042/bst0160087.
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