Kimura K
Hokkaido Igaku Zasshi. 1986 Jan;61(1):24-34.
Culture filtrates obtained from several isolates of Vibrio parahaemolyticus were tested for their enterotoxin-like activities. Also their antigenic relationship with cholera enterotoxin and Kanagawa hemolysin were tested. In the Chinese Hamster Ovary (CHO) cells assay which are usually employed for detecting cholera toxin or heat labile enterotoxin produced by enterotoxigenic Escherichia coli, crude culture filtrates obtained from both Kanagawa positive and negative strains isolated from patients showed positive reaction whereas most of the Kanagawa negative strains isolated from environmental sources did not. After concentration, however, both the culture filtrates of Kanagawa positive and negative strains of Vibrio parahaemolyticus showed positive reactions in CHO cell assay and rabbit skin permeability assay but not in rabbit ileal loop test. Antisera against concentrated culture filtrates obtained from both Kanagawa positive and negative strains neutralized neutralized the activities of concentrated culture filtrates in CHO cell assay and Rabbit permeability assay but not of cholera toxin and Kanagawa hemolysin. Immunodiffusion test was also carried out using the antisera and the concentrated culture filtrates obtained from both of Kanagawa positive and negative strains of Vibrio parahaemolyticus. The antisera cross-reacted with all concentrated culture filtrates obtained from both Kanagawa positive and negative strains but not reacted with cholera toxin and Kanagawa hemolysin. These results indicated that both of Kanagawa positive and negative strains of Vibrio parahaemolyticus can produce the enterotoxin-like factor(s) such as cholera enterotoxin or heat labile enterotoxin produced by enterotoxigenic Escherichia coli, but this factor(s) dose not show cross-antigenicity with cholera enterotoxin and the Kanagawa hemolysin produced by Kanagawa positive Vibrio parahaemolyticus.
对从几种副溶血性弧菌分离株获得的培养滤液进行了类肠毒素活性检测。还检测了它们与霍乱肠毒素和神奈川溶血素的抗原关系。在中国仓鼠卵巢（CHO）细胞检测中，通常用于检测霍乱毒素或产肠毒素大肠杆菌产生的热不稳定肠毒素，从患者分离的神奈川阳性和阴性菌株获得的粗培养滤液均呈阳性反应，而从环境来源分离的大多数神奈川阴性菌株则没有。然而，浓缩后，副溶血性弧菌神奈川阳性和阴性菌株的培养滤液在CHO细胞检测和兔皮肤通透性检测中均呈阳性反应，但在兔回肠袢试验中则没有。针对从神奈川阳性和阴性菌株获得的浓缩培养滤液的抗血清，在CHO细胞检测和兔通透性检测中中和了浓缩培养滤液的活性，但对霍乱毒素和神奈川溶血素没有作用。还使用抗血清和从副溶血性弧菌神奈川阳性和阴性菌株获得的浓缩培养滤液进行了免疫扩散试验。抗血清与从神奈川阳性和阴性菌株获得的所有浓缩培养滤液发生交叉反应，但与霍乱毒素和神奈川溶血素不发生反应。这些结果表明，副溶血性弧菌的神奈川阳性和阴性菌株均可产生类似霍乱肠毒素或产肠毒素大肠杆菌产生的热不稳定肠毒素的类肠毒素因子，但该因子与霍乱肠毒素和神奈川阳性副溶血性弧菌产生的神奈川溶血素不显示交叉抗原性。
