College of Chemistry and Materials, Fujian Normal University, Fuzhou, 350007, PR China.
College of Chemistry and Materials, Fujian Normal University, Fuzhou, 350007, PR China; Fujian Key Laboratory of Polymer Materials, Fuzhou, 350007, PR China; Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fuzhou, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Feb 15;227:117568. doi: 10.1016/j.saa.2019.117568. Epub 2019 Oct 17.
Although all kinds of sensors with unique detecting ability for one guest were reported, the fluorescence sensor with multiple detecting abilities was seldom presented. This work designed and synthesized a novel AIE fluorescence probe bearing double detecting for ATP based on Cu and Zn response of hydrazono-bis-tetraphenylethylene (Bis-TPE). Bis-TPE was prepared in 82% yield with simple procedure. It exhibited strong red AIE fluorescence based on the large conjugated electron effect in aqueous media. It showed outstanding selective sensing abilities for Cu by strong fluorescence quenching and for Zn by red-orange fluorescence change. The sensing mechanism of 1:1 stoichiometric ratios was confirmed by H NMR and MS study. The strong red fluorescence of Bis-TPE + Cu system could be recovered by adding ATP. The orange fluorescence of Bis-TPE + Zn system could be quenched by adding Cu and then was recovered by adding ATP. These double detecting abilities for ATP with the "off-on" red fluorescence in Bis-TPE + Cu system and "allochroic-off-on" orange fluorescence in Bis-TPE + Zn+Cu system were successfully applied to test Cu, Zn and ATP in test paper and living cell imaging, displaying the good application prospects for sensing Cu, Zn and double detecting ATP in the complicated environment.
尽管已经报道了各种具有独特检测能力的传感器，但具有多种检测能力的荧光传感器却很少见。本工作设计并合成了一种基于铜和锌对腙基双四苯乙烯（Bis-TPE）的响应的新型 AIE 荧光探针，用于检测 ATP。Bis-TPE 通过简单的步骤以 82%的收率制备，在水介质中具有基于大共轭电子效应的强红色 AIE 荧光。它对 Cu 表现出出色的选择性传感能力，通过强烈的荧光猝灭，对 Zn 则表现出红橙色荧光变化。通过 H NMR 和 MS 研究证实了 1:1 化学计量比的传感机制。Bis-TPE + Cu 体系的强红色荧光可以通过添加 ATP 恢复。Bis-TPE + Zn 体系的橙色荧光可以通过添加 Cu 猝灭，然后通过添加 ATP 恢复。Bis-TPE + Cu 体系中的“关-开”红色荧光和 Bis-TPE + Zn + Cu 体系中的“变构-关-开”橙色荧光的这种双重检测能力成功应用于试纸和活细胞成像中的 Cu、Zn 和 ATP 测试，显示出在复杂环境中用于检测 Cu、Zn 和双重检测 ATP 的良好应用前景。
