College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
School of Municipal and Environmental Engineering, Henan University of Urban Construction, Pingdingshan 467036, PR China.
Chemosphere. 2019 Jun;224:554-561. doi: 10.1016/j.chemosphere.2019.02.190. Epub 2019 Feb 28.
Hydrogen sulfide (HS), an important cellular signaling molecule, plays vital roles in mediating responses to biotic/abiotic stresses. Influences of HS on metal removal, cell viability, and antioxidant response of Phanerochaete chrysosporium upon exposure to heavy metals and silver nanoparticles (AgNPs) in the present study were investigated. An enhancement in Pb(ΙΙ) removal with an increase in concentration of the HS donor sodium hydrosulfide (NaHS) was observed, and the maximum removal efficiencies increased by 31% and 17% under 100 and 200 mg/L Pb(ΙΙ) exposure, respectively, in the presence of 500 μM NaHS. Application of 500 μM NaHS increased the cell viability by 15%-39% under Pb(II) stress (10-200 mg/L) with relative to the untreated control. Increase in total Ag uptake and cell survival was also elicited by NaHS in a concentration-dependent manner under AgNP stress. Meanwhile, activities of superoxide dismutase and catalase were significantly enhanced with the introduction of NaHS under stresses of Pb(II), Cd(II), Cu(II), Zn(II), Ni(II), and AgNPs. The inhibition in lipid peroxidation and oxidative stress was observed in P. chrysosporium cells exposed to these toxicants following NaHS pretreatment, which could be attributed to the upregulation in antioxidant enzymes. The results obtained suggest that HS can alleviate heavy metals and AgNP-induced toxicity to P. chrysosporium and improve the removal efficiency of these toxicants from wastewater.
硫化氢（HS）是一种重要的细胞信号分子，在介导生物/非生物胁迫响应方面发挥着重要作用。本研究探讨了 HS 对糙皮侧耳（Phanerochaete chrysosporium）在暴露于重金属和银纳米颗粒（AgNPs）时金属去除、细胞活力和抗氧化响应的影响。随着 HS 供体硫氢化钠（NaHS）浓度的增加，Pb(II) 的去除率得到增强，在 100 和 200mg/L Pb(II) 暴露下，最大去除效率分别提高了 31%和 17%，而在 500µM NaHS 存在下。与未处理的对照组相比，在 Pb(II) 胁迫（10-200mg/L）下，500µM NaHS 的应用使细胞活力提高了 15%-39%。在 AgNP 胁迫下，NaHS 也以浓度依赖的方式增加了总 Ag 摄取和细胞存活率。同时，在引入 NaHS 后，超氧化物歧化酶和过氧化氢酶的活性在 Pb(II)、Cd(II)、Cu(II)、Zn(II)、Ni(II) 和 AgNPs 的胁迫下显著增强。在 NaHS 预处理后，糙皮侧耳细胞暴露于这些有毒物质时，观察到脂质过氧化和氧化应激的抑制，这可能归因于抗氧化酶的上调。结果表明，HS 可以减轻重金属和 AgNP 对糙皮侧耳的毒性，并提高这些有毒物质从废水中的去除效率。
