Ravid A, Novogrodsky A, Wilchek M
Eur J Immunol. 1978 May;8(5):289-94. doi: 10.1002/eji.1830080502.
N4-Dinitrophenyl-L-2,4,-diaminobutyric acid hydrazide was coupled to aldehyde groups generated by periodate oxidation of sialyl residues on thymocytes. Anti-2,4-dinitrophenyl(Dnp)antibody was found to stimulate mature, hydrocortisone-resistant thymocytes, while it had no mitogenic effect on the immature thymocytes. In order to study the involvement of different regions of the antibody molecule in the triggering process of stimulation, the mitogenicity of various antibody fragments was also assessed. The divalent F(ab')2 was found to be a superior mitogen compared to the intact antibody when added to Dnp-conjugated thymocytes. The monovalent Fab' and Fab fragments have no mitogenic activity indicating that cross-linkage may be a prerequisite for stimulation.
N4-二硝基苯基-L-2,4-二氨基丁酸酰肼与经高碘酸盐氧化胸腺细胞上唾液酸残基产生的醛基偶联。发现抗2,4-二硝基苯基（Dnp）抗体可刺激成熟的、氢化可的松抗性胸腺细胞，而对未成熟胸腺细胞无促有丝分裂作用。为了研究抗体分子不同区域在刺激触发过程中的作用，还评估了各种抗体片段的促有丝分裂活性。当添加到Dnp偶联的胸腺细胞中时，发现二价F(ab')2 是比完整抗体更好的促有丝分裂原。单价Fab' 和Fab片段没有促有丝分裂活性，这表明交联可能是刺激的先决条件。
