Ciuffo M, Roggero P, Masenga V, Stravato V M
Istituto di Fitovirologia Applicata, C.N.R., Str. delle Cacce 73, I-10135 Torino, Italy.
Peto Italiana, Centro Ricerche di Latina, I-04010, B.go Sabotino, Latina, Italy.
Plant Dis. 1999 Jan;83(1):78. doi: 10.1094/PDIS.1999.83.1.78C.
Eggplant mottled dwarf rhabdovirus (EMDV) is endemic in the Mediterranean area but within the family Cucurbitaceae has been reported only in cucumber (1). In the spring of 1998 unusual symptoms of stunting, short internodes, fruit deformation, and vein yellowing were observed in about 5% of muskmelon (Cucumis melo L. var. reticulatus Naudin) cv. Hombre F grown under plastic in Tuscany (Central Italy). Electron microscopy of negatively stained preparations of crude sap from such melon plants revealed the presence of large numbers of particles resembling rhabdovirus. The virus, sap transmitted to several test plant species, had a host range identical to that of typical EMDV isolates, including the isolate from cucumber. Based on both electron microscopy and test plant reactions, the presence of other viruses was excluded. As with other known isolates, symptoms did not appear until about 30 days after inoculation, except for necrotic local lesions in Gomphrena globosa, which appeared in 10 days. Systemic leaf symptoms of field melon were reproduced by mechanically inoculating glasshouse melon seedlings with sap from infected White Burley tobacco. The virus was identified as EMDV by serology with an antiserum (As-0136) against the type isolate (EMDV-PV-0031), both obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ), Germany. No differences in protein pattern in Western blots (immunoblots) were observed by coelectrophoresis of the type isolate and our melon isolate. The virus was easily detected in several experimental hosts by antigen-coated plate enzyme-linked immunosorbent assay (ELISA). The vector of EMDV is unknown, but the virus generally infects in low percentage a wide range of plant species belonging to different families. This suggests that a polyphagous insect with low vector efficiency may be involved in transmission. Reference: (1) P. Roggero et al. Plant Dis. 79:321, 1995.
茄子斑驳矮化弹状病毒（EMDV）在地中海地区呈地方性流行，但在葫芦科内仅在黄瓜中被报道过（1）。1998年春季，在意大利中部托斯卡纳地区，约5%在塑料大棚中种植的网纹甜瓜（Cucumis melo L. var. reticulatus Naudin）品种Hombre F出现了生长迟缓、节间缩短、果实变形和叶脉黄化等异常症状。对这些甜瓜植株粗汁液的负染制剂进行电子显微镜观察，发现存在大量类似弹状病毒的颗粒。该病毒通过汁液传播到几种测试植物物种，其寄主范围与典型的EMDV分离株相同，包括来自黄瓜的分离株。基于电子显微镜观察和测试植物的反应，排除了其他病毒的存在。与其他已知分离株一样，除了在10天内出现坏死局部病斑的千日红外，接种后约30天症状才出现。用感染的白肋烟汁液机械接种温室甜瓜幼苗，再现了田间甜瓜的系统性叶片症状。通过与来自德国微生物和细胞培养物保藏中心（DSMZ）的针对典型分离株（EMDV-PV-0031）的抗血清（As-0136）进行血清学检测，该病毒被鉴定为EMDV。通过典型分离株和我们的甜瓜分离株的共电泳，在蛋白质印迹（免疫印迹）中未观察到蛋白质模式的差异。通过抗原包被板酶联免疫吸附测定（ELISA），很容易在几种实验寄主中检测到该病毒。EMDV的传播介体尚不清楚，但该病毒通常以低比例感染广泛的不同科植物物种。这表明可能涉及一种多食性但传毒效率低的昆虫参与传播。参考文献：（1）P. Roggero等人，《植物病害》79:321，1995年。
