Yuki V A, Rezende J A M, Kitajima E W, Barroso P A V, Kuniyuki H, Groppo G A, Pavan M A
Centro de Fitossanidade, Instituto Agronômico, 13020-902 Campinas, SP, Brazil.
(Fellows of CNPq), Dept. de Entomologia, Fitopatologia e Zoologia Agrícola, ESALQ/USP, 13418-900 Piracicaba, SP, Brazil.
Plant Dis. 1999 May;83(5):486. doi: 10.1094/PDIS.1999.83.5.486C.
Cayaponia tibiricae Cogn. (CT) is a wild Cucurbitaceae species found in secondary forests in the State of São Paulo, Brazil. The species has indefinite growth and bears oblong dark green fruits, 15 to 20 mm long (1,2). CT plants showing yellow mosaic symptoms were found in Atibaia County. Extracts from symptomatic plants were rub inoculated to zucchini squash (Cucurbita pepo L.) and Chenopodium amaranticolor Coste & Reyn. Zucchini squash plants developed severe yellow mosaic with intense leaf malformation, while C. amaranticolor showed necrotic local lesions. Extracts from naturally infected CT, zucchini squash, and C. amaranticolor were tested by plate trapped antigen-enzyme-linked immunosorbent assay (PTA-ELISA) with antisera against papaya ringspot virus type W (PRSV-W), zucchini yellow mosaic virus (ZYMV), zucchini lethal chlorosis virus (ZLCV), watermelon mosaic virus 2 (WMV-2), and cucumber mosaic virus (CMV). All samples were positive in PTA-ELISA only with ZYMV antiserum. Also, in Western blot (immunoblot) assay, ZYMV antiserum labeled a protein of approximately 36 kDa. Electron microscopic examination of ultrathin sections from infected CT tissue revealed the presence of pinwheel inclusions typical of potyvirus (type 1) infection in the cytoplasm of the cell. CT seedlings were susceptible to mechanical inoculation with the ZYMV isolated from this species. This is the first report of CT as a natural host for ZYMV in Brazil. References: (1) A. Cogniaux. Flora Brasiliensis 6:1, 1878. (2) M. Pio Corrêa. 1926. Diccionário das plantas úteis do Brasil e das exóticas cultivadas. Vol 1. Ministério da Agricultura, Rio de Janeiro, Brazil.
蒂比里卡卡亚波藤（Cayaponia tibiricae Cogn.，简称CT）是一种野生葫芦科植物，生长于巴西圣保罗州的次生林中。该物种生长无限，果实呈长圆形，深绿色，长15至20毫米（参考文献1,2）。在阿蒂巴亚县发现了表现出黄色花叶症状的CT植株。将有症状植株的提取物摩擦接种到西葫芦（Cucurbita pepo L.）和苋色藜（Chenopodium amaranticolor Coste & Reyn.）上。西葫芦植株出现严重的黄色花叶，并伴有严重的叶片畸形，而苋色藜则表现出坏死的局部病斑。用针对番木瓜环斑病毒W型（PRSV-W）、西葫芦黄花叶病毒（ZYMV）、西葫芦致死性褪绿病毒（ZLCV）、西瓜花叶病毒2型（WMV-2）和黄瓜花叶病毒（CMV）的抗血清，通过平板诱捕抗原-酶联免疫吸附测定（PTA-ELISA）对自然感染的CT、西葫芦和苋色藜的提取物进行检测。所有样品仅用ZYMV抗血清在PTA-ELISA中呈阳性。此外，在蛋白质印迹（免疫印迹）分析中，ZYMV抗血清标记出一条约36 kDa的蛋白质条带。对感染CT组织的超薄切片进行电子显微镜检查，发现在细胞的细胞质中存在典型的马铃薯Y病毒属（1型）感染的风轮状内含体。CT幼苗对从该物种分离出的ZYMV机械接种敏感。这是CT作为ZYMV在巴西的天然寄主的首次报道。参考文献：(1) A. Cogniaux. Flora Brasiliensis 6:1, 1878. (2) M. Pio Corrêa. 1926. Diccionário das plantas úteis do Brasil e das exóticas cultivadas. Vol 1. Ministério da Agricultura, Rio de Janeiro, Brazil.
