du Toit Lindsey J, Pataky Jerald K
Department of Crop Sciences, University of Illinois, Urbana 61801.
Plant Dis. 1999 Aug;83(8):727-732. doi: 10.1094/PDIS.1999.83.8.727.
Efforts at breeding for resistance to common smut of maize (Zea mays), caused by Ustilago maydis, are hampered by the lack of a reliable and efficient method of inoculation. Silk channel injection is one of the most efficient methods of inoculating for ear galls but is less consistent than acceptable for accurate assessment of the response of genotypes. The objective of this study was to examine how the silk channel inoculation method can be modified to reduce variation while maintaining efficiency for large-scale field inoculations. Variation associated with inoculum concentration and variation among people inoculating were examined. Incidence and severity of symptomatic ears increased with inoculum concentration. Concentrations between 10 and 10 sporidia/ml are recommended. Variation among people was greater than variation among inoculum concentrations. Incidence and severity ratings were lower for people inexperienced at inoculating with U. maydis than for experienced people. Variation among people inoculating can be controlled by appropriate experimental design.
由玉蜀黍黑粉菌（Ustilago maydis）引起的玉米普通黑粉病抗性育种工作，因缺乏可靠且高效的接种方法而受阻。花丝通道注射是诱发穗瘤最有效的接种方法之一，但在准确评估基因型反应方面，其一致性不如人意。本研究的目的是探讨如何改进花丝通道接种方法，以减少变异性，同时保持大规模田间接种的效率。研究了与接种物浓度相关的变异性以及接种人员之间的变异性。有症状果穗的发病率和严重程度随接种物浓度的增加而升高。建议使用浓度为每毫升10至10个担孢子的接种物。接种人员之间的变异性大于接种物浓度之间的变异性。与有经验的人员相比，缺乏用玉蜀黍黑粉菌接种经验的人员所记录的发病率和严重程度评级较低。接种人员之间的变异性可通过适当的实验设计加以控制。
