Department of Pathology, Leiden University Medical Center, 2333ZA Leiden, The Netherlands.
Department of Obstetrics and Gynaecology, Leiden University Medical Center, 2333ZA Leiden, The Netherlands.
Int J Mol Sci. 2019 Mar 6;20(5):1150. doi: 10.3390/ijms20051150.
In pregnancy, maternal physiology is subject to considerable adaptations, including alterations in cardiovascular and metabolic function as well as development of immunological tolerance towards the fetus. In an oocyte donation pregnancy, the fetus is fully allogeneic towards the mother, since it carries both oocyte donor antigens and paternal antigens. Therefore, oocyte donation pregnancies result in an immunologically challenging pregnancy, which is reflected by a higher-than-normal risk to develop pre-eclampsia. Based on the allogeneic conditions in oocyte donation pregnancies, we hypothesized that this situation may translate into alterations in concentration of stable readouts of constituents of the reactive species interactome (RSI) compared to normal pregnancies, especially serum free thiols, nitric oxide (NO) and hydrogen sulfide (H₂S) related metabolites. Indeed, total free thiol levels and nitrite (NO₂) concentrations were significantly lower whereas protein-bound NO and sulfate (SO₄) concentrations were significantly higher in both oocyte donation and naturally conceived pregnancies complicated by pre-eclampsia. The increased concentrations of nitrite observed in uncomplicated oocyte donation pregnancies suggest that endothelial NO production is compensatorily enhanced to lower vascular tone. More research is warranted on the role of the RSI and bioenergetic status in uncomplicated oocyte donation pregnancies and oocyte donation pregnancies complicated by pre-eclampsia.
在妊娠期间，母体生理学发生了很大的适应性变化，包括心血管和代谢功能的改变以及对胎儿的免疫耐受的发展。在卵母细胞捐赠妊娠中，胎儿对母亲完全是同种异体的，因为它携带卵母细胞供体抗原和父系抗原。因此，卵母细胞捐赠妊娠导致免疫挑战性妊娠，这表现为发生子痫前期的风险高于正常妊娠。基于卵母细胞捐赠妊娠中的同种异体条件，我们假设这种情况可能会导致反应性物质相互作用组（RSI）的组成部分的稳定读数浓度发生变化，与正常妊娠相比，特别是血清游离巯基、一氧化氮（NO）和硫化氢（H₂S）相关代谢物。事实上，总游离巯基水平和亚硝酸盐（NO₂）浓度在卵母细胞捐赠和自然受孕妊娠中均显著降低，而蛋白结合的 NO 和硫酸盐（SO₄）浓度在子痫前期合并卵母细胞捐赠和自然受孕妊娠中均显著升高。在未合并子痫前期的卵母细胞捐赠妊娠中观察到的亚硝酸盐浓度增加表明，内皮细胞 NO 生成是代偿性增强以降低血管张力。需要更多的研究来探讨 RSI 和生物能量状态在未合并子痫前期的卵母细胞捐赠妊娠和合并子痫前期的卵母细胞捐赠妊娠中的作用。
