Davidson M K, Sogn J A, Kazdin D S, Horng W J, Dray S, Gilman-Sachs A
J Immunol. 1986 May 15;136(10):3724-8.
We have shown that after immunization of homozygous a1 rabbits of the B immunoglobulin (Ig) heavy chain haplotype with anti-a2 antibody (Ab) a population of molecules appears that has all of the serologic characteristics of the a2 allotype. We have now isolated these putative latent a2 molecules, have separated the heavy chains, and after enzymatic deblocking, have determined the first 19 N-terminal amino acids. For all eight allotype-associated residues, these putative latent a2 molecules have the amino acid residues typical of a2 allotype. As expected, the preimmune IgG from this a1a1 rabbit has the amino acids typical of the a1 allotype. Thus by partial amino acid sequence analysis, we provide additional evidence that the latent a2 allotype can be induced in a1a1 rabbits of the B heavy chain haplotype by immunization with anti-a2 Ab. Rabbits of other heavy chain haplotypes were also immunized with anti-a2 Ab and were tested for their ability to synthesize latent a2 allotype. Thus far, a1a1 rabbits of the A, B, C, and I heavy chain haplotypes all synthesize latent a2 allotype. In contrast, a3a3 rabbits of the G and H heavy chain haplotypes did not synthesize latent a2 allotype.
我们已经表明，用抗a2抗体（Ab）免疫B免疫球蛋白（Ig）重链单倍型的纯合a1兔后，会出现一群具有a2同种异型所有血清学特征的分子。我们现在已经分离出这些假定的潜在a2分子，分离出重链，并在酶解去封闭后，确定了前19个N端氨基酸。对于所有八个与同种异型相关的残基，这些假定的潜在a2分子具有a2同种异型典型的氨基酸残基。正如预期的那样，这只a1a1兔的免疫前IgG具有a1同种异型典型的氨基酸。因此，通过部分氨基酸序列分析，我们提供了额外的证据，即通过用抗a2 Ab免疫，可以在B重链单倍型的a1a1兔中诱导出潜在的a2同种异型。其他重链单倍型的兔也用抗a2 Ab进行免疫，并测试它们合成潜在a2同种异型的能力。到目前为止，A、B、C和I重链单倍型的a1a1兔都能合成潜在的a2同种异型。相比之下，G和H重链单倍型的a3a3兔不能合成潜在的a2同种异型。
