通过 CRISPR/Cas9 文库筛选获得 rhEGF 生物测定用响应细胞
Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library.
机构信息
Department of Recombinant Products, National Institutes for Food and Drug Control, Beijing, 100050, China.
Department of Cell Engineering, Beijing Institute of Biotechnology, Beijing, 100850, China.
出版信息
Sci Rep. 2019 Mar 7;9(1):3780. doi: 10.1038/s41598-019-40381-4.
Bioassay of recombinant protein products is important tests to ensure protein effectiveness. Some recombinant protein products have no cells used in their bioassay but instead use animal models, while others have no suitable method. Here, we developed a method to obtain responsive cells used in bioassay of proteins. After screening of a CRISPR/Cas9 library, we obtained a responsive cell line that grew faster in the presence of rhEGF (recombinant human epidermal growth factor) than that of control cells. We used this cell line for bioassay of rhEGF. This cell line, compared with the control cells, had a 2 day shorter operation time and had lower interference. The responsive cell line is more suitable for use in bioassay of rhEGF.
重组蛋白产品的生物测定是确保蛋白质有效性的重要测试。一些重组蛋白产品在其生物测定中不使用细胞，而是使用动物模型，而另一些产品则没有合适的方法。在这里，我们开发了一种获得用于蛋白质生物测定的响应细胞的方法。在筛选了 CRISPR/Cas9 文库后，我们获得了一种响应细胞系，该细胞系在 rhEGF（重组人表皮生长因子）存在的情况下比对照细胞生长得更快。我们使用该细胞系进行 rhEGF 的生物测定。与对照细胞相比，该细胞系的操作时间缩短了 2 天，干扰更小。该响应细胞系更适合用于 rhEGF 的生物测定。
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