蛇血清的天然溶血活性。II. 假定天然抗体的异质性及溶血活性水平。

Natural hemolytic activity of snake serum. II. Heterogeneity of putative natural antibody and levels of hemolytic activity.

作者信息

Kawaguchi S, Muramatsu S, Mitsuhashi S

出版信息

Dev Comp Immunol. 1978 Jul;2(3):461-8. doi: 10.1016/s0145-305x(78)80007-x.

Abstract

The erythrocytes of various vertebrates, such as mice, rabbits, sheep, chickens, bullfrogs, and toads are lysed by normal snake sera. However, snake erythrocytes were not lysed by serum from different snake species. Putative natural antibody seems with different specificities to comprise heterogeneous antibodies. Thus, absorption of snake serum with mouse erythrocytes, for example, abrogated hemolytic activity for mice but not for rabbit or sheep erythrocytes. We observed no significant intraspecies individual differences in serum hemolytic titer, but interspecies differences were obvious. Immunization of snakes with sheep erythrocytes caused no further elevation of hemolytic activity, though high titer antibody was produced in response to certain bacterial antigens. Even the sera of newly-hatched snakes showed hemolytic activity at modestly high levels. No seasonal change in hemolytic activity was observed.

摘要

各种脊椎动物的红细胞，如小鼠、兔子、绵羊、鸡、牛蛙和蟾蜍的红细胞，会被正常蛇血清溶解。然而，蛇的红细胞不会被不同蛇种的血清溶解。推测天然抗体似乎由具有不同特异性的异质抗体组成。因此，例如用小鼠红细胞吸收蛇血清会消除对小鼠的溶血活性，但对兔子或绵羊红细胞则不会。我们观察到血清溶血效价在种内个体之间没有显著差异，但种间差异明显。用绵羊红细胞免疫蛇不会导致溶血活性进一步升高，尽管对某些细菌抗体会产生高滴度抗体。即使是刚孵化出的蛇的血清也显示出中等偏高水平的溶血活性。未观察到溶血活性的季节性变化。