Shaw C F, Schaeffer-Memmel N, Krawczak D
J Inorg Biochem. 1986 Mar;26(3):185-95. doi: 10.1016/0162-0134(86)80040-x.
The metabolites of gold in the urine of rats given the antiarthritic drug aurothiomalate were investigated by gel permeation chromatography, electrophoresis, and chemical studies. Following a single dose of aurtothiomalate, the excreted gold was protein-bound in the high-molecular-weight (greater than or equal to 150,000 dalton) and serum albumin fractions. Electrophoresis confirmed the presence of albumin, but showed that the other proteins present differ from those in normal or in vitro aurothiomalate-incubated rat sera. The pattern of the proteins establishes that the proteinuria was of the glomerular type. The alterations in the gold distribution produced by incubation of the urine with the low-molecular-weight thiol penicillamine and with exogenously added aurothiomalate indicated the existence of a labile equilibrium of gold among protein binding sites in the urine. Incubation of rat and human sera and commercially prepared serum albumins with aurothiomalate increased the electrophoretic mobility of the albumin. The significance of this change in electrophoretic mobility with respect to two models of gold binding by serum albumin is discussed.
采用凝胶渗透色谱法、电泳法和化学研究方法，对给予抗关节炎药物硫代苹果酸金钠的大鼠尿液中的金代谢产物进行了研究。单次给予硫代苹果酸金钠后，排泄出的金与高分子量（大于或等于150,000道尔顿）和血清白蛋白部分的蛋白质结合。电泳证实了白蛋白的存在，但显示存在的其他蛋白质与正常大鼠血清或体外经硫代苹果酸金钠孵育的大鼠血清中的蛋白质不同。蛋白质的模式表明蛋白尿属于肾小球型。用低分子量硫醇青霉胺和外源性添加的硫代苹果酸金钠孵育尿液所产生的金分布变化表明，尿液中蛋白质结合位点之间存在金的不稳定平衡。用硫代苹果酸金钠孵育大鼠和人血清以及商业制备的血清白蛋白会增加白蛋白的电泳迁移率。讨论了这种电泳迁移率变化相对于血清白蛋白结合金的两种模型的意义。
