Gil-Alonso S, Quindós G, Eraso E, Jauregizar N
Nerea Jauregizar, Department of Pharmacology (Unidad de formación e investigación multidisciplinar «Microbios, y Salud»), Faculty of Medicine and Nursing, University of Basque Country (UPV/EHU), Bilbao, Spain.
Rev Esp Quimioter. 2019 Apr;32(2):183-188. Epub 2019 Mar 8.
Candida albicans remains the most common aetiology of invasive candidiasis, leading to high morbidity and mortality. Nevertheless, the incidence of candidiasis due to non-C. albicans species, such as Candida parapsilosis, is increasing. Postantifungal effect (PAFE) is relevant for establishing dosage schedules in antifungal therapy, as the frequency of antifungal administration could change depending on PAFE. The aim of this study was to evaluate the PAFE of anidulafungin against C. albicans, Candida dubliniensis, Candida africana, C. parapsilosis, Candida metapsilosis and Candida orthopsilosis.
Twenty-one Candida strains were evaluated. Cells were exposed to anidulafungin for 1 h at concentrations ranging from 0.12 to 8 mg/L for PAFE studies. Time-kill experiments (TK) were conducted at the same concentrations. The experiments were performed using an inoculum of 1-5 x 105 cells/mL and 48 h incubation. Readings of PAFE and TK were done at 0, 2, 4, 6, 24 and 48 h.
Anidulafungin was fungicidal against 2 out of 14 (14%) strains of C. albicans related species in PAFE experiments. Moreover, 2 mg/L of anidulafungin exerted a prolonged PAFE (≥ 33.6 h) against 13 out of 14 (93%) strains. Similarly, fungicidal endpoint was achieved against 1 out of 7 (14%) strains of C. parapsilosis complex, being PAFE prolonged (≥ 42 h) against 6 out of 7 (86%) strains.
Anidulafungin induced a significant and prolonged PAFE against C. albicans and C. parapsilosis and their related species.
白色念珠菌仍是侵袭性念珠菌病最常见的病因，导致高发病率和死亡率。然而，由非白色念珠菌属物种，如近平滑念珠菌引起的念珠菌病发病率正在上升。抗真菌后效应（PAFE）对于确定抗真菌治疗的给药方案很重要，因为抗真菌药物的给药频率可能会根据PAFE而改变。本研究的目的是评估阿尼芬净对白色念珠菌、都柏林念珠菌、非洲念珠菌、近平滑念珠菌、近光滑念珠菌和正光滑念珠菌的PAFE。
评估了21株念珠菌菌株。为进行PAFE研究，将细胞暴露于浓度范围为0.12至8 mg/L的阿尼芬净中1小时。在相同浓度下进行时间杀菌实验（TK）。实验使用1-5×105个细胞/mL的接种物并孵育48小时。在0、2、4、6、24和48小时读取PAFE和TK数据。
在PAFE实验中，阿尼芬净对14株白色念珠菌相关菌株中的2株（14%）具有杀菌作用。此外，2 mg/L的阿尼芬净对14株菌株中的13株（93%）产生了延长的PAFE（≥33.6小时）。同样，对7株近平滑念珠菌复合体菌株中的1株（14%）达到了杀菌终点，对7株菌株中的6株（86%）的PAFE延长（≥42小时）。
阿尼芬净对白色念珠菌、近平滑念珠菌及其相关物种诱导了显著且延长的PAFE。
