Henderson K M, Prisk M D, Hudson N, Ball K, McNatty K P, Lun S, Heath D, Kieboom L E, McDiarmid J
J Reprod Fertil. 1986 Mar;76(2):623-35. doi: 10.1530/jrf.0.0760623.
Romney ewes were injected intramuscularly once or twice daily for 3 days with 0, 0.1, 0.5, 1 or 5 ml of bovine follicular fluid (bFF) treated with dextran-coated charcoal, starting immediately after injection of cloprostenol to initiate luteolysis on Day 10 of the oestrous cycle. There was a dose-related suppression of plasma concentrations of FSH, but not LH, during the treatment period. On stopping the bFF treatment, plasma FSH concentrations 'rebounded' to levels up to 3-fold higher than pretreatment values. The mean time to the onset of oestrus was also increased in a dose-related manner by up to 11 days. The mean ovulation rates of ewes receiving 1.0 ml bFF twice daily (1.9 +/- 0.2 ovulations/ewe, mean +/- s.e.m. for N = 34) or 5.0 ml once daily (2.0 +/- 0.2 ovulations/ewe, N = 25) were significantly higher than that of control ewes (1.4 +/- 0.1 ovulations/ewe, N = 35). Comparison of the ovaries of ewes treated with bFF for 24 or 48 h with the ovaries of control ewes revealed no differences in the number or size distribution of antral follicles. However, the large follicles (greater than or equal to 5 mm diam.) of bFF-treated ewes had lower concentrations of oestradiol-17 beta in follicular fluid, contained fewer granulosa cells and the granulosa cells had a reduced capacity to aromatize testosterone to oestradiol-17 beta and produce cyclic AMP when challenged with FSH or LH. No significant effects of bFF treatment were observed in small (1-2.5 mm diam.) or medium (3-4.5 mm diam.) sized follicles. Ewes receiving 5 ml bFF once daily for 27 days, from the onset of luteolysis, were rendered infertile during this treatment period. Oestrus was not observed and ovulation did not occur. Median concentrations of plasma FSH fell to 20% of pretreatment values within 2 days. Thereafter they gradually rose over the next 8 days to reach 60% of pretreatment values where they remained for the rest of the 27-day treatment period. Median concentrations of plasma LH increased during the treatment period to levels up to 6-fold higher than pretreatment values. When bFF treatment was stopped, plasma concentrations of FSH and LH quickly returned to control levels, and oestrus was observed within 2 weeks. The ewes were mated at this first oestrus and each subsequently delivered a single lamb.
在发情周期的第10天注射氯前列醇以启动黄体溶解后，立即对罗姆尼母羊每天进行1次或2次肌肉注射，持续3天，注射剂量分别为0、0.1、0.5、1或5毫升经葡聚糖包被活性炭处理的牛卵泡液（bFF）。在治疗期间，血浆FSH浓度呈现剂量依赖性抑制，但LH浓度未受影响。停止bFF治疗后，血浆FSH浓度“反弹”至比治疗前水平高出3倍的水平。发情开始的平均时间也以剂量依赖性方式增加，最长增加11天。每天两次接受1.0毫升bFF的母羊（1.9±0.2个排卵/母羊，N = 34时的平均值±标准误）或每天一次接受5.0毫升bFF的母羊（2.0±0.2个排卵/母羊，N = 25）的平均排卵率显著高于对照母羊（1.4±0.1个排卵/母羊，N = 35）。将接受bFF处理24或48小时的母羊卵巢与对照母羊卵巢进行比较，发现窦状卵泡的数量或大小分布没有差异。然而，bFF处理母羊的大卵泡（直径大于或等于5毫米）卵泡液中雌二醇-17β浓度较低，颗粒细胞数量较少，并且当受到FSH或LH刺激时，颗粒细胞将睾酮芳香化为雌二醇-17β并产生环磷酸腺苷的能力降低。在小（直径1 - 2.5毫米）或中（直径3 - 4.5毫米）卵泡中未观察到bFF处理的显著影响。从黄体溶解开始，每天一次接受5毫升bFF注射27天的母羊在此治疗期间不育。未观察到发情，也未发生排卵。血浆FSH的中位数浓度在2天内降至治疗前值的20%。此后，它们在接下来的8天内逐渐上升至治疗前值的60%，并在27天治疗期的剩余时间内保持在该水平。治疗期间血浆LH的中位数浓度增加至比治疗前水平高出6倍的水平。停止bFF治疗后，血浆FSH和LH浓度迅速恢复到对照水平，并且在2周内观察到发情。这些母羊在第一次发情时进行配种，随后每只母羊都产下一只羔羊。
