Vermassen Tijl, Callewaert Nico, Rottey Sylvie, Delanghe Joris R
Department of Medical Oncology, Ghent University Hospital, Ghent, Belgium.
Drug Research Unit Ghent, Ghent University Hospital, Ghent, Belgium.
Methods Mol Biol. 2019;1972:235-250. doi: 10.1007/978-1-4939-9213-3_17.
DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis allows for accurate profiling of the asparagine-linked (N-) glycosylation patterns, a posttranslational modification present on many soluble and membrane proteins. This technique has been extensively tested to identify N-glycosylation patterns associated with serum proteins. Here we describe the use of DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis to identify the N-glycosylation patterns of prostate proteins in urine.
DNA测序仪辅助的荧光团辅助碳水化合物电泳可实现对天冬酰胺连接（N-）糖基化模式的精确分析，这是一种存在于许多可溶性和膜蛋白上的翻译后修饰。该技术已得到广泛测试，用于识别与血清蛋白相关的N-糖基化模式。在此，我们描述了使用DNA测序仪辅助的荧光团辅助碳水化合物电泳来识别尿液中前列腺蛋白的N-糖基化模式。
