Starr C M, Masada R I, Hague C, Skop E, Klock J C
Glyko, Novato, CA 94949, USA.
J Chromatogr A. 1996 Jan 12;720(1-2):295-321. doi: 10.1016/0021-9673(95)00749-0.
Carbohydrate analysis has traditionally been viewed as a specialty science, performed only in a few well-established laboratories using conventional carbohydrate analysis technology (e.g. NMR, gas chromatography-mass spectroscopy, high-performance liquid chromatography, capillary electrophoresis) combined with the specialized technical training that has been essential for accurate interpretation of the data. This tradition of specialized laboratories is changing, due primarily to an increase in the number of scientists performing routine carbohydrate analysis. As a result, many scientists who are not trained in traditional carbohydrate analytical techniques now need to be able to perform accurate carbohydrate analysis in their own laboratories. This has created a need for technically simple and inexpensive methods of carbohydrate analysis. In this review, we present application vignettes of a technically simple, yet analytically powerful method called fluorophore-assisted carbohydrate electrophoresis (FACE). FACE can be used for performing routine oligosaccharide profiling, monosaccharide analysis, and sequencing of a variety of carbohydrates.
传统上，碳水化合物分析被视为一门专业科学，只有少数设备完善的实验室采用传统的碳水化合物分析技术（如核磁共振、气相色谱-质谱联用、高效液相色谱、毛细管电泳）并结合专业技术培训来进行，而这种专业技术培训对于准确解读数据至关重要。由于进行常规碳水化合物分析的科学家数量增加，这种由专业实验室主导的传统正在发生变化。因此，许多未接受过传统碳水化合物分析技术培训的科学家现在需要能够在自己的实验室中进行准确的碳水化合物分析。这就产生了对技术简单且成本低廉的碳水化合物分析方法的需求。在本综述中，我们展示了一种名为荧光团辅助碳水化合物电泳（FACE）的技术简单但分析能力强大的方法的应用示例。FACE可用于进行常规寡糖分析、单糖分析以及多种碳水化合物的测序。
