Cannon G W, Cole B C, Ward J R
J Rheumatol. 1986 Feb;13(1):52-7.
The effects of in vitro gold sodium thiomalate (GSTM) on peripheral blood mononuclear cells (PBM) stimulated by a Mycoplasma arthritidis T cell mitogen (MAM), concanavalin A (Con A), and phytohemagglutinin (PHA) were compared in 5 individuals by measuring 3H-thymidine uptake by PBM. The MAM induced lymphocyte transformations were more significantly inhibited by in vitro GSTM than parallel assays using Con A and PHA. The effects could not be explained by differences in cell viability, length of cell culture, mitogen concentration, and level of mitogen stimulation, suggesting that MAM may be a useful probe in evaluating the effects of GSTM on immune function.
通过测量外周血单个核细胞（PBM）对³H-胸腺嘧啶核苷的摄取，比较了体外硫代苹果酸金钠（GSTM）对关节炎支原体T细胞有丝分裂原（MAM）、刀豆蛋白A（Con A）和植物血凝素（PHA）刺激的5名个体外周血单个核细胞（PBM）的影响。与使用Con A和PHA的平行试验相比，体外GSTM对MAM诱导的淋巴细胞转化的抑制作用更为显著。这些作用无法用细胞活力、细胞培养时间、有丝分裂原浓度和有丝分裂原刺激水平的差异来解释，这表明MAM可能是评估GSTM对免疫功能影响的有用探针。
