Crary B, Borysenko M, Sutherland D C, Kutz I, Borysenko J Z, Benson H
J Immunol. 1983 Feb;130(2):694-7.
A single subcutaneous injection of 0.2 mg epinephrine into healthy human subjects caused a transient lymphocytosis in peripheral blood. Mononuclear cells (MNC), isolated at various times after epinephrine administration, were cultured in the presence of mitogens. The blastogenic responses to pokeweed mitogen (PWM) and phytohemagglutinin (PHA) were significantly reduced for up to 60 min post-epinephrine (p less than 0.05); the response to concanavalin A (Con A) was reduced in the 15-min samples only. All responses returned to pre-injection levels by 120 min post-injection. Removal of adherent monocytes from MNC isolates before culture did not restore normal mitogen responsiveness. When MNC were cultured in the absence of mitogens, there was no difference in survival between pre- and post-epinephrine samples. Incubation of untreated MNC for 2 hr or 18 hr in vitro with various concentrations of epinephrine (10(-5) to 10(-1) mg/ml) had no effect upon the subsequent blastogenic response to mitogens. Other workers have reported that epinephrine administration causes alterations in the composition of the circulating lymphocyte pool. Taken together, these data suggest that the reduction in mitogen responsiveness after epinephrine is the result of changes in the distribution of lymphocyte subclasses in peripheral blood.
对健康人体受试者进行一次皮下注射0.2毫克肾上腺素，会导致外周血出现短暂的淋巴细胞增多。在注射肾上腺素后的不同时间分离出单核细胞（MNC），并在有丝分裂原存在的情况下进行培养。肾上腺素注射后长达60分钟，对商陆有丝分裂原（PWM）和植物血凝素（PHA）的增殖反应显著降低（p小于0.05）；仅在15分钟的样本中，对刀豆球蛋白A（Con A）的反应降低。注射后120分钟时，所有反应均恢复到注射前水平。在培养前从MNC分离物中去除黏附单核细胞并不能恢复正常的有丝分裂原反应性。当MNC在无有丝分裂原的情况下培养时，肾上腺素注射前后样本的存活率没有差异。用不同浓度的肾上腺素（10^(-5)至10^(-1)毫克/毫升）在体外对未处理的MNC孵育2小时或18小时，对随后对有丝分裂原的增殖反应没有影响。其他研究人员报告称，注射肾上腺素会导致循环淋巴细胞池的组成发生改变。综合来看，这些数据表明，肾上腺素注射后有丝分裂原反应性降低是外周血淋巴细胞亚群分布变化的结果。
