1 Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
2 Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
J Interferon Cytokine Res. 2019 Jun;39(6):347-354. doi: 10.1089/jir.2018.0116. Epub 2019 Mar 8.
Behçet's disease (BD) is a chronic multi-systemic inflammatory disease of uncertain pathogenesis and with no definitive diagnostic test. The aims of this study were to investigate serum levels of miR-181b in BD patients and to correlate this candidate biomarker with disease activity, cytokines, and adhesion molecules to identify new markers that can be used as a diagnostic tool for BD. Blood samples were collected from 96 participants who were classified according to their BD current activity form into 3 groups: healthy control, active BD, and inactive BD patients. MiR-181b was estimated by real-time polymerase chain reaction. However, high sensitive C-reactive protein (hs-CRP), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), E-selectin, and vascular cell adhesion molecule 1 (VCAM-1) levels were determined by enzyme-linked immunosorbent assay. Serum levels of miR-181b, hs-CRP, TNF-α, IL-6, E-selectin, and VCAM-1 were significantly higher in patients than in controls, but no significant difference was observed between the active and inactive BD groups. IL-6 was positively correlated with adhesion molecules, E-selectin, and VCAM-1. MiR-181b was positively correlated with hs-CRP, TNF-α, IL-6, and VCAM-1 in all subjects. In conclusion, miR-181b could play an important role in BD pathophysiology. MiR-181b could be utilized as potential biomarker for diagnosis and therapeutic targeting of BD. However, further studies with larger patient number are required to support these findings.
贝赫切特病（BD）是一种病因不明的慢性多系统炎症性疾病，没有明确的诊断测试。本研究旨在探讨 BD 患者血清 miR-181b 水平，并将该候选生物标志物与疾病活动、细胞因子和黏附分子相关联，以确定可用于 BD 诊断的新标志物。采集了 96 名参与者的血液样本，根据其 BD 当前活动形式将其分为 3 组：健康对照组、活动期 BD 患者组和非活动期 BD 患者组。采用实时聚合酶链反应（PCR）测定 miR-181b 水平。然而，通过酶联免疫吸附试验（ELISA）测定高敏 C 反应蛋白（hs-CRP）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、E-选择素和血管细胞黏附分子 1（VCAM-1）水平。与对照组相比，患者的血清 miR-181b、hs-CRP、TNF-α、IL-6、E-选择素和 VCAM-1 水平均显著升高，但活动期和非活动期 BD 组之间无显著差异。IL-6 与黏附分子 E-选择素和 VCAM-1 呈正相关。在所有受试者中，miR-181b 与 hs-CRP、TNF-α、IL-6 和 VCAM-1 呈正相关。综上所述，miR-181b 在 BD 发病机制中可能发挥重要作用。miR-181b 可作为潜在的生物标志物，用于 BD 的诊断和治疗靶向。然而，需要更多的包含更大患者数量的研究来支持这些发现。
