Mesquida Marina, Molins Blanca, Llorenç Victor, Sainz de la Maza Maite, Hernandez María Victoria, Espinosa Gerard, Adán Alfredo
Institut Clínic d'Oftalmologia, Hospital Clinic de Barcelona, University of Barcelona, Sabino de Arana 1, 08028 Barcelona, Spain.
Institut Clínic d'Oftalmologia, Hospital Clinic de Barcelona, University of Barcelona, Sabino de Arana 1, 08028 Barcelona, Spain ; Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
Mediators Inflamm. 2014;2014:396204. doi: 10.1155/2014/396204. Epub 2014 Jun 8.
The aim of the present study was to determine the serum cytokine profile and levels of high sensitivity C-reactive protein (hsCRP) in patients with uveitis associated with Behçet's disease (BD) and to compare them with those obtained from healthy control subjects. We determined the serum concentration of interferon-γ (IFN-γ), interleukin-1β (IL-1β), IL-12p70, IL-17A, tumor necrosis factor-α (TNF-α), and hsCRP in 13 patients with active uveitis associated to BD, 24 inactive BD patients, and 20 controls. In a subgroup of 10 active patients, a second serum sample was obtained when the disease was inactive. Cytokine profiles and hsCRP levels were correlated with disease activity, severity, complications, and visual outcome. Levels of IFN-γ and TNF-α were significantly increased in patients with active uveitis associated to BD compared to controls (P < 0.05). IFN-γ, TNF-α, and hsCRP were significantly higher during active uveitis associated to BD compared to inactive disease (P < 0.05). Furthermore, IL-17A was significantly increased in patients with active BD without pharmacological treatment compared to controls (P < 0.05). No significant correlations were found with specific cytokine profiles and disease severity, visual outcome, or complications. In summary, increased serum levels of IFN-γ, TNF-α, IL-17A, and hsCRP were associated with active uveitis associated with BD and might serve as markers of disease activity.
本研究的目的是确定白塞病（BD）相关葡萄膜炎患者的血清细胞因子谱和高敏C反应蛋白（hsCRP）水平，并将其与健康对照者的水平进行比较。我们测定了13例活动性BD相关葡萄膜炎患者、24例非活动性BD患者和20例对照者血清中干扰素-γ（IFN-γ）、白细胞介素-1β（IL-1β）、IL-12p70、IL-17A、肿瘤坏死因子-α（TNF-α）和hsCRP的浓度。在10例活动性患者的亚组中，当疾病处于非活动期时采集了第二份血清样本。细胞因子谱和hsCRP水平与疾病活动度、严重程度、并发症及视力预后相关。与对照组相比，活动性BD相关葡萄膜炎患者的IFN-γ和TNF-α水平显著升高（P<0.05）。与非活动性疾病相比，活动性BD相关葡萄膜炎期间IFN-γ、TNF-α和hsCRP显著更高（P<0.05）。此外，与对照组相比，未接受药物治疗的活动性BD患者的IL-17A显著升高（P<0.05）。未发现特定细胞因子谱与疾病严重程度、视力预后或并发症之间存在显著相关性。总之，血清中IFN-γ、TNF-α、IL-17A和hsCRP水平升高与BD相关活动性葡萄膜炎有关，可能作为疾病活动的标志物。
