Environmental Research and Education Center, University of São Paulo, CEPEMA-POLI-USP, Cônego Domênico Rangoni Rd, 270 km, Cubatão-SP, Brazil.
Metallomics. 2019 May 22;11(5):949-958. doi: 10.1039/c8mt00365c.
Metal contamination exerts environmental pressure on several lifeforms. Since metals are non-biodegradable and recalcitrant, they accumulate in living beings and spread through the food chain. Thus, many life forms are affected by environmental metal contamination, such as plants and microorganisms. In the case of microorganisms, scarce information is available on how metals affect them. As a highly resistant form of life, microorganisms can adapt to several environmental pressures through genetic modifications, changing their metabolism to overcome new conditions, and continuing to thrive in the same place. In this study, an Acinetobacter sp. strain was isolated from a copper mine, which presented very high resistance to copper, growing in copper concentrations of up to 7 mM. As a result of its metabolic response in the presence of 3 mM of copper, the expression of 35 proteins in total was altered. The proteins were identified to be associated with the glycolytic pathway, membrane transport, biosynthesis and two proteins directly involved in copper homeostasis (CopA and CopB).
金属污染对多种生物形式施加了环境压力。由于金属是不可生物降解和顽固的，它们在生物体内积累并通过食物链传播。因此，许多生命形式受到环境金属污染的影响，如植物和微生物。就微生物而言，关于金属如何影响它们的信息很少。作为一种高度抗逆的生命形式，微生物可以通过遗传修饰来适应多种环境压力，改变它们的新陈代谢以克服新的条件，并继续在同一地方茁壮成长。在这项研究中，从铜矿中分离出一株不动杆菌属菌株，该菌株对铜具有很高的抗性，在高达 7mM 的铜浓度下生长。由于其在存在 3mM 铜时的代谢反应，总共改变了 35 种蛋白质的表达。这些蛋白质被鉴定与糖酵解途径、膜转运、生物合成以及直接参与铜稳态的两种蛋白质（CopA 和 CopB）有关。
