Department of Parasitology, Faculty of Veterinary Medicine, Erciyes University, 38280, Kayseri, Turkey.
Vectors and Vector-Borne Diseases Implementation and Research Center, Erciyes University, 38280, Kayseri, Turkey.
J Eukaryot Microbiol. 2019 Sep;66(5):771-777. doi: 10.1111/jeu.12725. Epub 2019 Mar 24.
Microsporidia are opportunistic pathogens that infect a wide range of invertebrates and vertebrates. To assess the potential role of dogs in the transmission of these zoonotic pathogens, a total of 282 fecal samples from dogs in the Central Anatolia Region of Turkey were analyzed by utilizing species specific polymerase chain reaction for the four most frequent human microsporidia. Two microsporidia species were recognized in 41 samples (14.5%). Encephalitozoon intestinalis was detected in 35 samples (12.4%) and it was the most common microsporidium. The second microsporidium, E. cuniculi, was identified in six (2.1%) of the samples. Sequence analysis of the intergenic spacer of the ribosomal ribonucleic acid (RNA) internal transcribed spacer (ITS) gene revealed the presence of three E. intestinalis haplotypes closely associated with each other. No polymorphic region was found among the ITS sequences of E. cuniculi isolates and they were characterized as genotype III. This study provides the first data on the zoonotic microsporidia species from dogs in Turkey.
微孢子虫是机会性病原体，可感染广泛的无脊椎动物和脊椎动物。为了评估狗在这些人畜共患病原体传播中的潜在作用，对土耳其中安纳托利亚地区的 282 份狗粪便样本进行了分析，利用针对四种最常见的人类微孢子虫的种特异性聚合酶链反应。在 41 份样本（14.5%）中识别出两种微孢子虫。在 35 份样本（12.4%）中检测到肠微孢子虫，这是最常见的微孢子虫。第二种微孢子虫，E. cuniculi，在 6 份样本（2.1%）中被鉴定出来。核糖体核糖核酸（RNA）内部转录间隔区（ITS）基因的基因间 spacer 的序列分析显示，存在三种与彼此密切相关的 E. intestinalis 单倍型。在 E. cuniculi 分离株的 ITS 序列中未发现多态性区域，它们被表征为基因型 III。本研究提供了土耳其狗的人畜共患病微孢子虫种的首次数据。
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