Kalantari Mohsen, Azizi Kourosh, Junaghani Negin Kiani, Vahedi Mozaffar, Mohammadpour Iraj, Asgari Qasem, Hosseinpour Amin, Miri Mehdi
Research Center for Health Sciences, Institute of Health, Department of Biology and Control of Disease Vectors, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
Student Research Committee, Department of Biology and Control of Disease Vectors, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
Parasite Epidemiol Control. 2025 Jul 1;30:e00446. doi: 10.1016/j.parepi.2025.e00446. eCollection 2025 Aug.
Microsporidia, particularly and , are emerging fungal pathogens of global concern, causing severe gastrointestinal and systemic infections in immunocompromised individuals. Cockroaches (Blattodea), as synanthropic pests, are potential mechanical vectors for disseminating these pathogens in urban environments. Despite their clinical significance, data on the role of cockroaches in transmitting microsporidia in arid regions remain scarce. This study assessed the contamination of cockroaches in Shiraz, Iran, with and to evaluate their public health risks. From January to June 2023, 378 cockroaches were collected from high-risk areas, including Hospitals and adjacent public areas. Specimens were morphologically identified and screened via microscopy. Molecular detection of and was performed using PCR targeting the 18sRNA region. DNA was extracted from pooled samples (10 cockroaches/pool) and amplified under standardized thermocycling conditions. All cockroaches were identified as (77.5 %) and (22.5 %). In molecular assay, three positive case of was detected, However, no DNA was identified via PCR. This study reports the first molecular detection of in Shiraz cockroaches. In continue, it records no evidence of , which suggesting climatic or environmental barriers to limit this microsporidian persistence in this arid region. These findings highlight the need for integrated vector management and advanced molecular surveillance to elucidate the epidemiology of understudied pathogens like microsporidia in rapidly urbanizing regions.
微孢子虫，尤其是[具体种类1]和[具体种类2]，是引起全球关注的新兴真菌病原体，可在免疫功能低下的个体中引起严重的胃肠道和全身感染。蟑螂（蜚蠊目）作为共生害虫，是在城市环境中传播这些病原体的潜在机械传播媒介。尽管它们具有临床意义，但关于蟑螂在干旱地区传播微孢子虫作用的数据仍然很少。本研究评估了伊朗设拉子市蟑螂被[具体种类1]和[具体种类2]污染的情况，以评估其公共卫生风险。2023年1月至6月，从包括医院和相邻公共区域在内的高风险地区收集了378只蟑螂。通过显微镜对标本进行形态学鉴定和筛查。使用针对18sRNA区域的PCR对[具体种类1]和[具体种类2]进行分子检测。从合并样本（每组10只蟑螂）中提取DNA，并在标准化热循环条件下进行扩增。所有蟑螂均被鉴定为[具体种类1]（77.5%）和[具体种类2]（22.5%）。在分子检测中，检测到3例[具体种类1]阳性病例，然而，通过PCR未鉴定出[具体种类2]的DNA。本研究报告了在设拉子蟑螂中首次对[具体种类1]进行分子检测。接着，该研究没有记录到[具体种类2]的证据，这表明存在气候或环境障碍限制了这种微孢子虫在这个干旱地区的持续存在。这些发现强调了需要进行综合病媒管理和先进的分子监测，以阐明在快速城市化地区像微孢子虫这样研究不足的病原体的流行病学。
