Li Jun, Wang Zhi-Guang, Pang Long-Bin, Zhang Rong-Hua, Wang Ya-Yan
a Department of respiratory medicine , Jinan Center Hospital Affiliated to Shandong University , Jinan , Shandong , P.R. China.
b Department of Respiratory Medicine , Affiliated Hospital of Yanbian University , Yanji , Jilin , P.R. China.
Biosci Biotechnol Biochem. 2019 Jun;83(6):1077-1084. doi: 10.1080/09168451.2019.1588094. Epub 2019 Mar 8.
CENPU (centromere protein U), a centromere component essential for mitosis, relates with some cancers progression. However, it is not well illustrated in lung adenocarcinoma (LAC). Here, we aimed to investigate the potential effect of CENPU on LAC progression and prognosis. In this experiment, expression level of CENPU and association between its expression and LAC patients' clinicopathological characteristics and prognosis were analyzed. The proliferation, migration and invasive abilities of LAC cells were determined by CCK-8, colony formation, transwell assays. Western blot was used to detect PI3K/AKT signaling key proteins. We found CENPU level was overexpressed in LAC tissues on comparing normal tissues. Moreover, CENPU overexpression correlated with clinicopathological variables and predicted an independent prognostic indicator in LAC patients. Functionally, CENPU downregulation significantly inhibited LAC cell proliferation, migration and invasion in, which was possibly mediated by PI3K/AKT pathway inactivation. Our findings insinuate targeting CENPU may be a potential therapeutic strategy for LAC.
着丝粒蛋白U（CENPU）是有丝分裂所必需的着丝粒成分，与某些癌症进展相关。然而，其在肺腺癌（LAC）中的作用尚未得到充分阐明。在此，我们旨在研究CENPU对LAC进展和预后的潜在影响。在本实验中，分析了CENPU的表达水平及其表达与LAC患者临床病理特征和预后的相关性。通过CCK-8、集落形成、Transwell实验检测LAC细胞的增殖、迁移和侵袭能力。采用蛋白质免疫印迹法检测PI3K/AKT信号通路关键蛋白。我们发现，与正常组织相比，LAC组织中CENPU水平过表达。此外，CENPU过表达与临床病理变量相关，并可作为LAC患者独立的预后指标。在功能上，CENPU下调显著抑制LAC细胞增殖、迁移和侵袭，这可能是由PI3K/AKT通路失活介导的。我们的研究结果表明，靶向CENPU可能是LAC的一种潜在治疗策略。
