Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, B-9052 Ghent, Belgium; VIB-UGent Center for Plant Systems Biology, Technologiepark 927, B-9052 Ghent, Belgium.
Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, B-9052 Ghent, Belgium; VIB-UGent Center for Plant Systems Biology, Technologiepark 927, B-9052 Ghent, Belgium.
Curr Opin Biotechnol. 2019 Oct;59:16-23. doi: 10.1016/j.copbio.2019.02.002. Epub 2019 Mar 5.
Metabolic pathways are tightly regulated at the transcriptional and post-translational level, often relying on protein-protein interactions or post-translational protein modifications. Whereas these principles have been established already for a long time, the number of experimentally established cases is expected to rise exponentially in the near future as a result of recent advances in protein-based detection methods. Interactions and modifications are often dependent on only short amino-acid sequences that represent excellent targets for new gene editing technologies by which specific base pairs can be exchanged. Here, we introduce the concept of metabolic editing, which is based on identifying specific amino-acid sequences that are subsequently targeted for gene editing. The proposed workflow will serve for both applied metabolic engineering purposes and proof-of-concept studies in fundamental research.
代谢途径在转录和翻译后水平受到严格调控，通常依赖于蛋白质-蛋白质相互作用或翻译后蛋白质修饰。尽管这些原则已经确立了很长时间，但由于基于蛋白质的检测方法的最新进展，预计在不久的将来，实验确定的案例数量将呈指数级增长。相互作用和修饰通常仅依赖于短的氨基酸序列，这些序列是新的基因编辑技术的绝佳靶标，通过该技术可以交换特定的碱基对。在这里，我们介绍了代谢编辑的概念，该概念基于识别随后针对基因编辑进行靶向的特定氨基酸序列。所提出的工作流程将适用于应用代谢工程目的和基础研究中的概念验证研究。
