Deknudt G
Mutat Res. 1986 May;174(1):67-70. doi: 10.1016/0165-7992(86)90078-3.
Frequencies of sister-chromatid exchanges (SCE) were determined in second-division metaphases of human lymphocytes, exposed for 1 h during the G0 phase to mitomycin C (MMC) alone or to cyclophosphamide (CP) in the presence of S9 mix. The cells were then cultured for 72 h in the presence of phytohemagglutinin (PHA), concanavalin A (Con A), Wistaria floribunda (WFA) or Lens culinaris (LcH-A) extracts. Large differences in mitotic indices (MI) and cell-cycle kinetics were observed among cells subjected to the various treatments. However, in the controls as well as in the cultures submitted to a G0 mutagenic exposure, the yield of SCE was not influenced by the mitogenic agent and was, therefore, independent of the proliferation properties of the cultured lymphocyte population.
在人淋巴细胞的第二次分裂中期测定姐妹染色单体交换（SCE）频率，细胞在G0期单独暴露于丝裂霉素C（MMC）1小时，或在S9混合物存在下暴露于环磷酰胺（CP）1小时。然后，细胞在植物血凝素（PHA）、刀豆球蛋白A（Con A）、紫藤（WFA）或扁豆（LcH-A）提取物存在下培养72小时。在接受各种处理的细胞中观察到有丝分裂指数（MI）和细胞周期动力学存在很大差异。然而，在对照以及接受G0诱变暴露的培养物中，SCE的产量不受促有丝分裂剂的影响，因此，与培养的淋巴细胞群体的增殖特性无关。
