多种促细胞分裂剂对化学物质诱导的人淋巴细胞姐妹染色单体交换产率的影响。

Influence of various mitogens on the yield of sister-chromatid exchanges, induced by chemicals, in human lymphocytes.

作者信息

Deknudt G, Kamra O

出版信息

Mutat Res. 1983 Oct;111(2):161-70. doi: 10.1016/0027-5107(83)90060-x.

Abstract

The fluorochrome-plus-Giemsa (FPG) method of Perry and Wolff was used to compare the frequencies of sister-chromatid exchanges (SCEs) induced by cyclophosphamide (CP) or mitomycin C (MMC) in human lymphocytes stimulated by phytohaemagglutinin (PHA), concanavalin A (Con A), Wistaria floribunda (WFA), or lentil lectin (LcH-A) extracts. These 4 mitogens, differing in lectin valency and/or sugar specificity, are considered as activating primarily thymus-derived (T) lymphocytes. Regardless of the mitogen used, control cultures displayed a mean yield of about 8 SCEs/cell. A contact, of 1 h, with mitomycin alone or with cyclophosphamide and enzymatic activation, resulted in a significant augmentation of SCEs dependent on the mitogen used. An approximately 2-fold, 4-fold, or 6-fold increase in SCEs was observed for the cultures stimulated by PHA, Con A, and WFA or LcH-A respectively. Furthermore, there were mitogen-dependent differences in mitotic indices and cell-cycle kinetics in human lymphocytes harvested 72 h after stimulation.

摘要

采用佩里和沃尔夫的荧光染料加吉姆萨（FPG）方法，比较在植物血凝素（PHA）、刀豆球蛋白A（Con A）、紫藤凝集素（WFA）或小扁豆凝集素（LcH-A）提取物刺激的人淋巴细胞中，环磷酰胺（CP）或丝裂霉素C（MMC）诱导的姐妹染色单体交换（SCE）频率。这4种有丝分裂原在凝集素价和/或糖特异性方面存在差异，被认为主要激活胸腺来源的（T）淋巴细胞。无论使用何种有丝分裂原，对照培养物的平均产率约为8个SCE/细胞。与单独的丝裂霉素或与环磷酰胺接触1小时并进行酶促激活，会导致依赖于所用有丝分裂原的SCE显著增加。分别观察到PHA、Con A以及WFA或LcH-A刺激的培养物中SCE增加约2倍、4倍或6倍。此外，在刺激72小时后收获的人淋巴细胞中，有丝分裂指数和细胞周期动力学存在有丝分裂原依赖性差异。