Deknudt G, Kamra O
Mutat Res. 1983 Oct;111(2):161-70. doi: 10.1016/0027-5107(83)90060-x.
The fluorochrome-plus-Giemsa (FPG) method of Perry and Wolff was used to compare the frequencies of sister-chromatid exchanges (SCEs) induced by cyclophosphamide (CP) or mitomycin C (MMC) in human lymphocytes stimulated by phytohaemagglutinin (PHA), concanavalin A (Con A), Wistaria floribunda (WFA), or lentil lectin (LcH-A) extracts. These 4 mitogens, differing in lectin valency and/or sugar specificity, are considered as activating primarily thymus-derived (T) lymphocytes. Regardless of the mitogen used, control cultures displayed a mean yield of about 8 SCEs/cell. A contact, of 1 h, with mitomycin alone or with cyclophosphamide and enzymatic activation, resulted in a significant augmentation of SCEs dependent on the mitogen used. An approximately 2-fold, 4-fold, or 6-fold increase in SCEs was observed for the cultures stimulated by PHA, Con A, and WFA or LcH-A respectively. Furthermore, there were mitogen-dependent differences in mitotic indices and cell-cycle kinetics in human lymphocytes harvested 72 h after stimulation.
采用佩里和沃尔夫的荧光染料加吉姆萨（FPG）方法，比较在植物血凝素（PHA）、刀豆球蛋白A（Con A）、紫藤凝集素（WFA）或小扁豆凝集素（LcH-A）提取物刺激的人淋巴细胞中，环磷酰胺（CP）或丝裂霉素C（MMC）诱导的姐妹染色单体交换（SCE）频率。这4种有丝分裂原在凝集素价和/或糖特异性方面存在差异，被认为主要激活胸腺来源的（T）淋巴细胞。无论使用何种有丝分裂原，对照培养物的平均产率约为8个SCE/细胞。与单独的丝裂霉素或与环磷酰胺接触1小时并进行酶促激活，会导致依赖于所用有丝分裂原的SCE显著增加。分别观察到PHA、Con A以及WFA或LcH-A刺激的培养物中SCE增加约2倍、4倍或6倍。此外，在刺激72小时后收获的人淋巴细胞中，有丝分裂指数和细胞周期动力学存在有丝分裂原依赖性差异。
