Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, PR China; Shanxi Key Laboratory of Active ConstituentsＲesearch and Utilization of TCM, Taiyuan 030006, PR China.
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, PR China; Shanxi Key Laboratory of Active ConstituentsＲesearch and Utilization of TCM, Taiyuan 030006, PR China.
J Pharm Biomed Anal. 2019 May 30;169:99-110. doi: 10.1016/j.jpba.2019.02.041. Epub 2019 Feb 26.
Psychological suboptimal health state (PSHS), a subtype of suboptimal health status (SHS), seriously threatens the physical and mental health of human beings. Baihe Dihuang Tang (BDT), a traditional Chinese medicine prescription, has been used to improve PSHS in clinical and achieve significant efficacy for a long time. Exploring of the underlying mechanism of BDT improving the state of PSHS is of significant importance. In the present work, all subjects were screened in strict accordance with inclusion and exclusion criteria. the UHPLC-Q Exactive Orbitrap-MS and Trace GC-PolarisQ Mass were performed to analyze the metabolic features of BDT improving the state of PSHS. Combined with the experimental results of metabolomics and the predicted results of network pharmacology, the metabolic biological network was constructed to find the potential targets of BDT intervention on PSHS. Finally, A total of 22 differential metabolites have been identified in PSHS group. 15 plasma biomarkers were significantly regulated by BDT. The results indicated that the BDT decoction is of a significant therapeutic effect on the improvement of PSHS primarily through regulating pyruvate metabolism and phenylalanine metabolism. Moreover, it is possible for BDT to improve PSHS through the functional targets including GLO1, MAOA and MAOB, which are closely related to monoamine neurotransmitters. Here, these approaches provide a tractable, powerful tool for understanding the underlying mechanism elucidating of BDT for PSHS management.
心理亚健康状态（PSHS）是亚健康状态（SHS）的一种亚型，严重威胁着人类的身心健康。百合地黄汤（BDT）作为一种中药方剂，长期以来一直被用于改善 PSHS，并取得了显著疗效。探索 BDT 改善 PSHS 状态的潜在机制具有重要意义。在本工作中，所有受试者均严格按照纳入和排除标准进行筛选。采用 UHPLC-Q Exactive Orbitrap-MS 和 Trace GC-PolarisQ Mass 对 BDT 改善 PSHS 的代谢特征进行分析。结合代谢组学实验结果和网络药理学预测结果，构建代谢生物网络，寻找 BDT 干预 PSHS 的潜在靶点。最终，在 PSHS 组中鉴定出 22 个差异代谢物。BDT 显著调节 15 种血浆生物标志物。结果表明，BDT 对 PSHS 的改善具有显著的治疗作用，主要通过调节丙酮酸代谢和苯丙氨酸代谢。此外，BDT 可能通过与单胺神经递质密切相关的功能靶点 GLO1、MAOA 和 MAOB 来改善 PSHS。这些方法为理解 BDT 改善 PSHS 的潜在机制提供了一种可行的、强大的工具。
