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蜘蛛美洲草蛛中的卵黄蛋白原——卵黄发生的表达谱及假定的激素调控

Vitellogenins in the spider Parasteatoda tepidariorum - expression profile and putative hormonal regulation of vitellogenesis.

作者信息

Bednarek Agata W, Sawadro Marta K, Nicewicz Łukasz, Babczyńska Agnieszka I

机构信息

Department of Animal Physiology and Ecotoxicology, University of Silesia, Bankowa 9, PL40007, Katowice, Poland.

出版信息

BMC Dev Biol. 2019 Mar 8;19(1):4. doi: 10.1186/s12861-019-0184-x.

DOI:10.1186/s12861-019-0184-x
PMID:30849941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6408786/
Abstract

BACKGROUND

Knowledge about vitellogenesis in spiders is rudimentary. Therefore, the aim of study was to check the vitellogenin (Vg) presence in various tissues of the female spider Parasteatoda tepidariorum, determine when and where vitellogenesis starts and takes place, and the putative role of selected hormones in the vitellogenesis.

RESULTS

Here we show two genes encoding Vg (PtVg4 and PtVg6) in the genome of the spider P. tepidariorum. One gene PtVg4 and three subunits of Vg (250 kDa, 47 kDa and 30 kDa) are expressed in the midgut glands, ovaries and hemolymph. Heterosynthesis of the Vg in the midgut glands and autosynthesis in the ovaries were observed. Vitellogenesis begins in the last nymphal stage in the midgut glands (heterosynthesis). However, after sexual maturity is reached, Vg is also synthesized in the ovaries (autosynthesis). Changes in the PtVg4 expression level and in the Vg concentration after treatment with 20-hydroxyecdysone, a juvenile hormone analog (fenoxycarb) and an antijuvenoid compound (precocene I) were observed. Therefore, we propose a hypothetical model for the hormonal regulation of vitellogenesis in P. tepidariorum.

CONCLUSIONS

Our results are the first comprehensive study on spider vitellogenesis. In our opinion, this work will open discussion on the evolutionary context of possible similarities in the hormonal control of vitellogenesis between P. tepidariorum and other arthropods as well as their consequences.

摘要

背景

关于蜘蛛卵黄发生的知识尚不完善。因此，本研究的目的是检测温血拟壁钱蛛（Parasteatoda tepidariorum）雌蛛不同组织中卵黄原蛋白（Vg）的存在情况，确定卵黄发生开始的时间和位置，以及所选激素在卵黄发生中的假定作用。

结果

我们在温血拟壁钱蛛的基因组中发现了两个编码Vg的基因（PtVg4和PtVg6）。一个基因PtVg4以及Vg的三个亚基（250 kDa、47 kDa和30 kDa）在中肠腺、卵巢和血淋巴中表达。观察到中肠腺中Vg的异源合成和卵巢中的自体合成。卵黄发生始于末龄若虫阶段的中肠腺（异源合成）。然而，性成熟后，Vg也在卵巢中合成（自体合成）。观察了用20-羟基蜕皮激素、一种保幼激素类似物（烯虫酯）和一种抗保幼激素化合物（早熟素I）处理后PtVg4表达水平和Vg浓度的变化。因此，我们提出了一个温血拟壁钱蛛卵黄发生激素调节的假设模型。

结论

我们的结果是对蜘蛛卵黄发生的首次全面研究。我们认为，这项工作将开启关于温血拟壁钱蛛与其他节肢动物在卵黄发生激素控制方面可能存在的相似性及其后果的进化背景的讨论。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/43567f367bf1/12861_2019_184_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/e86351230afe/12861_2019_184_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/a28785fa86c9/12861_2019_184_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/de2c491ce812/12861_2019_184_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/7aa42e75be28/12861_2019_184_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/20f8ca5289ba/12861_2019_184_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/43567f367bf1/12861_2019_184_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/e86351230afe/12861_2019_184_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/a28785fa86c9/12861_2019_184_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/de2c491ce812/12861_2019_184_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/7aa42e75be28/12861_2019_184_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/20f8ca5289ba/12861_2019_184_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/6408786/43567f367bf1/12861_2019_184_Fig6_HTML.jpg

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