The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Department of Oral Implantology, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, 510055, Guangzhou, People's Republic of China.
Clin Oral Investig. 2019 Nov;23(11):4133-4143. doi: 10.1007/s00784-019-02852-w. Epub 2019 Mar 9.
Osteoimmune interactions possess a critical part in the integration of materials and hosts. Dendritic cells (DCs) are the most common members of osteoimmune cells family. The titanium surfaces of dental implants tend to promote a mature dendritic cell phenotype with increased proinflammatory secretion. However, very little is known to the effects of this microenvironment on the behaviors of cells around implants, especially osteoblasts, and how the tissue integrations take place on such biomaterial surfaces. Therefore, the present study was to investigate the interactions of DCs with osteoblasts on titanium surfaces. DCs seeded on PT and SLA titanium surfaces were compared by assays for the proliferations, surface markers, and inflammatory genes expressions.
DCs seeded on PT and SLA titanium surfaces were compared by assays for the proliferations, surface markers, and inflammatory genes expressions. Next, we harvested the dendritic cell-conditioned medium (CM) and investigated the effects of CM on MC3T3-E1.
The results showed an increase in CD86 and proinflammatory expressions of DCs seeded on PT and SLA surfaces, as well as a decrease in anti-inflammatory cytokines. The CM from titanium surfaces inhibited the osteoblast differentiation with reduced expression of osteogenic genes RUNX2, COL1, ALP, and OCN and decreased ALP activity as well as Alizarin red staining.
These findings suggested that titanium surfaces switch DCs toward maturation phenotypes and thus inhibit the differentiation and mineralization of osteoblasts.
Knowing the significant impact of immune cells on osteogenesis behaviors, some efforts to decrease the immune reaction might be of clinical significance. Favorable immune environments can increase the dental implants survival rate in patients.
骨免疫相互作用在材料和宿主的整合中起着关键作用。树突状细胞（DCs）是骨免疫细胞家族中最常见的成员。牙种植体的钛表面往往促进具有增加的促炎分泌的成熟树突状细胞表型。然而，对于这种微环境对植入物周围细胞（尤其是成骨细胞）的行为的影响以及组织整合如何在这种生物材料表面发生，知之甚少。因此，本研究旨在研究 DCs 与钛表面上的成骨细胞的相互作用。通过增殖、表面标志物和炎症基因表达的测定比较了接种在 PT 和 SLA 钛表面上的 DCs。
通过增殖、表面标志物和炎症基因表达的测定比较了接种在 PT 和 SLA 钛表面上的 DCs。接下来，我们收集树突状细胞条件培养基（CM）并研究 CM 对 MC3T3-E1 的影响。
结果显示，接种在 PT 和 SLA 表面上的 DCs 的 CD86 和促炎表达增加，抗炎细胞因子减少。来自钛表面的 CM 抑制成骨细胞分化，降低成骨基因 RUNX2、COL1、ALP 和 OCN 的表达，并降低碱性磷酸酶活性和茜素红染色。
这些发现表明，钛表面使 DCs 向成熟表型转变，从而抑制成骨细胞的分化和矿化。
了解免疫细胞对成骨行为的重大影响，减少免疫反应可能具有临床意义。有利的免疫环境可以提高患者中牙科植入物的存活率。
