土耳其牛诺如病毒和内博病毒的分子特征：重组毒株的检测
Molecular characterization of bovine noroviruses and neboviruses in Turkey: detection of recombinant strains.
作者信息
Karayel-Hacioglu Ilke, Alkan Feray
机构信息
Department of Virology, Ankara University, Faculty of Veterinary Medicine, Diskapi, 06110, Ankara, Turkey.
出版信息
Arch Virol. 2019 May;164(5):1411-1417. doi: 10.1007/s00705-019-04186-0. Epub 2019 Mar 8.
To investigate the molecular epidemiology and genetic diversity of bovine enteric caliciviruses, a total of 167 fecal samples from diarrheic calves were screened. Bovine noroviruses (BoNoVs) and neboviruses were detected in 56 (33.5%) and 37 (22.1%) fecal samples, respectively. Sequences of the RdRp and capsid gene of selected BoNoVs showed that the GIII.1 and GIII.2 genotypes were in circulation in Turkey. Two of the BoNoV strains were identified as recombinant strains (GIII.P1/GIII.2). All examined neboviruses possessed a Nebraska-like RdRp gene. The two nebovirus strains were classified into lineage 4 based on phylogenetic analysis of VP1 amino acid sequences. One of them showed evidence of a recombination event within the S domain. This study is thus the first to reveal the presence of the BoNoV GIII.1 genotype and recombinant strains of BoNoV and neboviruses in Turkey.
为了研究牛肠道杯状病毒的分子流行病学和遗传多样性，对167份腹泻犊牛的粪便样本进行了筛查。分别在56份（33.5%）和37份（22.1%）粪便样本中检测到牛诺如病毒（BoNoVs）和内布拉斯加病毒。所选BoNoVs的RdRp和衣壳基因序列显示，GIII.1和GIII.2基因型在土耳其流行。其中两株BoNoV菌株被鉴定为重组菌株（GIII.P1/GIII.2）。所有检测的内布拉斯加病毒都拥有类似内布拉斯加的RdRp基因。根据VP1氨基酸序列的系统发育分析，两株内布拉斯加病毒菌株被分类为第4分支。其中一株在S结构域内显示出重组事件证据。因此，本研究首次揭示了土耳其存在BoNoV GIII.1基因型以及BoNoV和内布拉斯加病毒的重组菌株。
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